Assessment of luteolin isolated from Eclipta alba leaves in animal models of epilepsy

Figures & data

Table 1. Effect of luteolin on motor coordination in rotarod model.

Treatment	Latency to fall from rotarod
Control (CMC)	299 ± 0.91
Diazepam 2 mg/kg	84.33 ± 19.87***
Luteolin 5 mg/kg	300 ± 0.00
Luteolin 10 mg/kg	293.33 ± 2.43
Luteolin 20 mg/kg	297.5 ± 2.28

The data are expressed as mean ± SEM (n = 6) and were analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test. Differences were considered to be statistically significant when

*** p < 0.001 compared to normal control.

Table 2. Effect of luteolin in PTZ-induced seizure test.

	Time in seconds
Treatments	Onset of myoclonic jerks	Onset of clonic seizures	Onset of HLE	Onset of death	% Protectionfrom death
PTZ Control	50.14 ± 2.71	57.17 ± 1.13	122.34 ± 9.20	223.17 ± 22.46	0
Diazepam 2 mg/kg	612.11 ± 11.42***	0 ± 0.00	0 ± 0.00	0 ± 0.00	100
Luteolin 5 mg/kg	69.17 ± 3.86	79.67 ± 5.65	112.5 ± 17.44	543.25 ± 24.15***	66.66
Luteolin 10 mg/kg	169.49 ± 16.58***	214.10 ± 34.76**	238.25 ± 18.67*	0 ± 0.00	100
Luteolin 20 mg/kg	267.51 ± 10.84***	536.12 ± 34.34**	672.67 ± 57.16***	0 ± 0.00	100

The data are expressed as mean ± SEM (n = 6) and were analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test. Differences were considered to be statistically significant when

* p < 0.05,

** p < 0.01,

*** p < 0.001 compared to PTZ control.

Figure 1. Effect of luteolin on seizure score in PTZ-induced kindling model. The data are expressed as mean ± SEM (n = 10) and were analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test. Differences were considered to be statistically significant when *p < 0.05, **p < 0.01, ***p < 0.001 compared to PTZ control.

Figure 2. Effect of luteolin on MDA levels in PTZ kindling model. The data are expressed as mean ± SEM (n = 6) and were analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test. Differences were considered to be statistically significant when *p < 0.05, **p < 0.01, ***p < 0.001 compared to PTZ control and ^@p < 0.001 when compared to normal control.

Figure 3. Effect of luteolin on reduced GSH levels in PTZ kindling model. The data are expressed as mean ± SEM (n = 6) and were analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test. Differences were considered to be statistically significant when *p < 0.05, **p < 0.01, ***p < 0.001 compared to PTZ control and ^@p < 0.001 when compared to normal control.