Immunomodulatory activity of Buchholzia coriacea seed methanol extract on Trypanosoma brucei brucei infected mice

Figures & data

Table 1. Effect of methanolic extract of Buchholzia coriacea seeds on delayed hypersensitivity reaction mice estimated as paw swelling (mm).

Treatment	Dose (mg/kg)	Paw swelling (mm)	Inhibition (%)
Extract	250	0.84 ± 0.22^a	20.9
Extract	500	0.84 ± 0.07^a	20.8
Extract	1000	2.14 ± 0.02^b	−101.9
Levamisole	7.5	1.06 ± 0.09^a	0
Control	–	1.06 ± 0.09^a	–

Mean ± SEM with different superscript letters are significantly different (p < 0.05).

Table 2. Effect of methanolic extract of Buchholzia coriacea seeds on the parasitaemia (10⁶ parasites/ml) before and after treatment.

Treatment	Dose (mg/kg)	Day 0 of the treatment Parasites (10^6)	Day 8 of the treatment Parasites (10^6)
Extract	250	22.38 ± 42321.09	28.32 ± 43425^a
Extract	500	19.02 ± 81782.12	18.09 ± 109934^b
Extract	1000	20.87 ± 11223	16.82 ± 98852^b
Levamisole	7.5	34.98 ± 87822.65	35.12 ± 21345^a
Control	–	29.23 ± 34245	89.93 ± 23424^c

Mean ± SEM with different superscript letters are significantly different (p < 0.05).

Table 3. Effect of methanolic extract of Buchholzia coriacea seeds on primary and secondary antibody response (log₁₀2) in mice.

Treatment	Dose (mg/kg)	Primary antibody	Secondary antibody
Extract	250	3.20 ± 1.36	3.20 ± 1.36^a
Extract	500	3.20 ± 0.49	8.00 ± 3.58^a
Extract	1000	5.60 ± 2.64	6.80 ± 2.50^a
Levamisole	7.5	2.80 ± 0.80	6.80 ± 3.77^a
Control	–	1.20 ± 0.49	1.60 ± 0.40^b

Mean ± SEM with different superscript letters are significantly different (p < 0.05).

Table 4. Effect of Buchholzia coriacea seed extract on total and differential leucocytes mobilization (cells/ml) in mice.

	250 (mg/kg)	500 (mg/kg)	1000 (mg/kg)	Levamisole	Control
TLC	1450.00 ± 0.22	1430 ± 367.29	1670 ± 371.01	1110.00 ± 167.63	950.00 ± 184.3
NEUT	279.80 ± 70.59	362.60 ± 104.53	397.40 ± 84.72	277.80 ± 69.35	182.00 ± 733.40
LYMPH	1149.40 ± 369.63	1042.80 ± 265.98	1303.80 ± 100.52	826.40 ± 103.85	733.40 ± 144.79
BASO	5.80 ± 5.80^a	0.00 ± 0.00^a	0.00 ± 0.00^a	1.60 ± 0.60^a	16.60 ± 3.49^b
MONO	10.20 ± 2.34^ab	1.40 ± 0.40^a	17.80 ± 4.91^b	1.60 ± 0.60^a	18.00 ± 3.27^b
EOSIN	0.00 ± 0.00	5.80 ± 0.50	5.80 ± 0.55	0.00 ± 0.00	6.40 ± 2.91

Mean ± SEM with different superscript letters are significantly different (p < 0.05).