Bioactivity guided isolation of cytotoxic terpenoids and steroids from Premna serratifolia

Figures & data

Figure 1. TLC of PS-01 MLR with markers.

Figure 2. TLC of PS-02 A.

Figure 3. LCMS of PS-02 A.

Figure 4. Cytotoxicity of the isolates on cell lines.

Table 1. Cytotoxicity of the isolated compounds/fractions by BSL assay.

		Mean percent death after 24 h (concentration in μg/ml)
Fraction code	Fraction name	10	100	1000	LC50 (PPM)
Control	Normal	0.00	0.00	0.00	0.00
Standard	Potassium dichromate	40	100	100	11.50
PS-01A	Oleanolic acid	23.33 ± 0.33	53.33 ± 0.33	96.67 ± 0.33	54.49
PS-01B	Stigmasterol	36.67 ± 0.33	66.67 ± 0.33	76.67 ± 0.33	30.83
PS-02A	Unknown	40	83.33 ± 0.33	100	16.32

Cytotoxicity effects (mean percent death after 24 h with LC₅₀ values) of the plants are expressed in terms of mean ± S.E.M. of three replicates of samples.

Table 2. Cytotoxicity of the isolated compounds/fractions on cell lines.

Compound	IC50 (PPM)
	A549	HepG2	L6
PS-01A	90.53	69.57	228.59
PS-01B	82.76	74.29	160.20
PS-02A	66.77	53.72	112.93

The compounds/fractions with IC₅₀ values ≤100 μg/ml were considered as active.