Hepatoprotective effect of Stachys pilifera ethanol extract in carbon tetrachloride-induce hepatotoxicity in rats

Figures & data

Table 1. Effect of of Stachys pilifera ethanol extract on liver function tests in CCl₄-induced liver toxicity in rats.

Treatment group	ALT (U/l)	AST (U/l)	ALP (U/l)	TP (g/dL)	ALB (g/dL)
Control	77 ± 6	147 ± 11	308 ± 33	6.9 ± 0.31	3.8 ± 0.13
CCl4	167 ± 14***	222 ± 15***	800 ± 54***	6.23 ± 0.081***	2.7 ± 0.31***
100 S.P + CCl4	122 ± 14***###	222 ± 6***	695 ± 63***##	6.65 ± 0.15##	2.85 ± 0.22*
200 S.P + CCl4	97 ± 8*###	193 ± 14.6***###	519 ± 34***###	6.98 ± 0.14###	2.95 ± 0.18
400 S.P + CCl4	79 ± 3###	160 ± 8###	382 ± 20**###	7.1 ± 0.25###	2.76 ± 0.37
400 S.P only	70 ± 10###	157 ± 8###	362 ± 39###	7.08 ± 0.14###	4.01 ± 0.25

Values are presented as Mean ± S.D. S.P: ethanolic extract of Stachy spilifera; ALT: alanine aminotransferase; ALP: alkaline phosphatase; AST: aspartate aminotransferase; ALB: albumin; TP: total protein.

* p < 0.05.

** p < 0.01.

*** p < 0.001 vs. control group.

## p < 0.01.

### p < 0.001 vs. CCl₄ group, n = 7.

Figure 1. Effect of Stachys pilifera ethanol extract on the serum MDA level in CCl₄-induced hepatotoxicity in rats. Data are expressed as Mean ± S.D. *p < 0.05 and ***p < 0.001 vs. control group. C, p < 0.001 vs. CCl₄ group, n = 7.

Figure 2. H&E staining of liver tissues isolated from CCl₄-exposed rats demonstrated that CCl₄ poisoning resulted in excessive formation of fatty acid, necrosis of Kupffer cells around the central vein and netrophil infilteration (B). The ethanol extract at the doses of 200 and 400 mg/kg/d reversed the liver injures by CCl₄, towards normal pattern (C and D). The extract at the dose of 100 mg/kg/d did not show a considerable protective effect on the pathological changes induced by CCl₄ (E). Examination of the liver sections from the rat treated only with 400 mg/kg/d Stachys pilifera showed normal architecture (F) similar to control group (A).