1,770
Views
9
CrossRef citations to date
0
Altmetric
Research Article

Anti-inflammatory activity of standardized dichloromethane extract of Salvia connivens on macrophages stimulated by LPS

, , , , &
Pages 1467-1472 | Received 22 Jun 2016, Accepted 05 Mar 2017, Published online: 27 Mar 2017

Figures & data

Figure 1. Structures of (a) ursolic acid, (b) oleanolic acid, (c) dihydroursolic acid, and (d) eupatorin.

Figure 1. Structures of (a) ursolic acid, (b) oleanolic acid, (c) dihydroursolic acid, and (d) eupatorin.

Figure 2. Chromatograms of the ursolic acid reference (a) and DESC solution (b).

Figure 2. Chromatograms of the ursolic acid reference (a) and DESC solution (b).

Figure 3. Cell viability in macrophages treated with DESC at 1, 5, 10, 25, 50, 100 and 200 μg/ml, as determined by the MTT assay. Results are expressed as the percentage of surviving cells relative to control cells. The results are the mean of three determinations ± SEM.

Figure 3. Cell viability in macrophages treated with DESC at 1, 5, 10, 25, 50, 100 and 200 μg/ml, as determined by the MTT assay. Results are expressed as the percentage of surviving cells relative to control cells. The results are the mean of three determinations ± SEM.

Figure 4. Effects of the DESC extract on the LPS-stimulated levels of (a) TNF-α, (b) IL-1β, (c) IL-6 and (d) IL-10 levels in macrophages. The concentrations were determined by ELISA. The results are the mean of three determinations ± SEM. * p < 0.05 vs. LPS group.

Figure 4. Effects of the DESC extract on the LPS-stimulated levels of (a) TNF-α, (b) IL-1β, (c) IL-6 and (d) IL-10 levels in macrophages. The concentrations were determined by ELISA. The results are the mean of three determinations ± SEM. * p < 0.05 vs. LPS group.