Antioxidant and anti-inflammatory activities of Aerva pseudotomentosa leaves

Figures & data

Figure 1. TLC profile of Aerva pseudotomentosa aqueous extract.

Figure 2. HPTLC fingerprint of Aerva pseudotomentosa aqueous extract.

Table 1. HPTLC profile of aqueous extract of Aerva pseudotomentosa.

Peak	Rf	Height	Area
1	0.02	20.0	184.8
2	0.12	16.6	366.4
3	0.16	18.0	445.3
4	0.29	28.1	1151.5
5	0.50	18.7	506.6
6	0.64	387.9	14465.7
7	0.71	462.1	13911.0
8	0.97	40.8	645.8

Figure 3. (a) HPLC chromatogram of reference standard rutin (retention time 11.427). (b) HPLC chromatogram of APAE (retention time 11.423).

Table 2. Effect of APAE on DPPH radical scavenging.

Concentration (μg/mL)	% DPPH Inhibition	IC50 Value
APAE
20	30.57 ± 1.18	49.37 μg/mL
40	48.62 ± 0.79
60	58.36 ± 0.67
80	66.31 ± 2.51
100	73.89 ± 0.43
BHT
2	36.76 ± 1.73	4.22 μg/mL
4	50.25 ± 1.04
6	61.15 ± 0.96
8	68.51 ± 0.24
10	78.68 ± 0.36

Values are mean ± SEM, (n = 3), IC₅₀ = 50% Inhibitory concentration.

Figure 4. Effect of APAE on reducing potential.

Table 3. Effect of APAE on H₂O₂ scavenging.

Concentration (μg/mL)	% Inhibition	IC50 Value
APAE
100	15.05 ± 0.53	288.20 μg/mL
200	32.93 ± 1.07
300	52.55 ± 1.74
400	65.56 ± 2.79
500	95.30 ± 1.34
Ascorbic acid
20	37.99 ± 2.40	52.75 μg/mL
40	46.02 ± 2.60
60	51.12 ± 0.86
80	60.36 ± 3.24
100	67.96 ± 1.84

Values are mean ± SEM, (n = 3), IC₅₀ = 50% Inhibitory concentration.

Figure 5. Anti-lipid peroxidation (LPO) assay in Flax seed homogenate.

Table 4. Anti-inflammatory effect of APAE on carrageenan-induced rat paw oedema.

	Increase in paw volume
Groups	1st h	3rd h	5th	% Inhibition at 5th h
Control	0.262 ± 0.008	0.630 ± 0.017	0.802 ± 0.044
INDO 10 mg/kg	0.195 ± 0.036	0.315 ± 0.040#	0.313 ± 0.056#	60.97
APAE 200 mg/kg	0.237 ± 0.055	0.447 ± 0.044	0.503 ± 0.037@	37.28
APAE 400 mg/kg	0.203 ± 0.033	0.390 ± 0.053*	0.437 ± 0.071#	45.11

Results were expressed in mean ± SEM (n = 5) #p < 0.001, @p < 0.01 compared to control, *p < 0.05.

Table 5. Anti-inflammatory effect of APAE on formalin-induced rat paw oedema.

Groups	Increase in paw volume
	1st h	3rd h	5th h	% Inhibition at 5th h
Control	0.184 ± 0.014	0.301 ± 0.01	0.518 ± 0.033
INDO 10 mg/kg	0.173 ± 0.011	0.156 ± 0.011#	0.241 ± 0.008#	53.47
APAE 200 mg/kg	0.197 ± 0.019	0.255 ± 0.021	0.283 ± 0.025#	45.36
APAE 400 mg/kg	0.179 ± 0.013	0.163 ± 0.015#	0.262 ± 0.027#	49.42

Results were expressed in mean ± SEM (n = 5). #p < 0.001 compared to control.