Antimicrobial, modulatory and chemical analysis of the oil of Croton limae

Figures & data

Table 1. Chemical constituents of the essential oil of C. limae leaves.

Constituents	RT (min)	RI	(%)
α-Pinene	5.2	939	13.8
β-Pinene	6.2	980	3.0
β-Myrcene	6.8	991	1.5
p-Cymene	7.7	1026	4.2
Eucalyptol	7.8	1033	17.4
Linalool	10.1	1098	2.5
Cryptone	12.8	1186	1.3
p-Cymen-8-ol	12.8	1189	1.3
β-Caryophyllene	20.1	1418	3.8
α-Humulene	21.1	1452	2.3
Alloaromadendrene	21.2	1458	1.2
Spathulenol	22.9	1576	2.8
Caryophyllene oxide	23.7	1581	1.2
Cedrol	24.9	1589	28.4
Total identified			84.7

RT: retention time; RI: Kovats index (Adams 2001).

Table 2. MIC of the essential oil of C. limae against the standard bacterial strains.

Bacteria	MIC (μg/mL)
Escherichia coli ATCC 10536	≥1024
Klebsiella pneumoniae ATCC 4362	≥1024
Pseudomonas aeruginosa ATCC 15442	≥1024
Staphylococcus aureus ATCC 25923	512

Table 3. Modulatory activity of the essential oil of C. limae against the multiresistant bacterial strains.

	Staphylococcus aureusSA 358		Escherichia coli EC 27
Antibiotic	Control	EOCL (μg/mL)	Control	EOCL (μg/mL)
Amikacin	19.53	9.76	312.5	39.062
Gentamicin	2.44	2.44	39.062	39.062
Neomycin	9.76	9.76	78.125	2.44

EOCL: essential oil of Croton limae.

Table 4. MIC of the essential oil of C. limae against the standard fungi strains.

Fungi	MIC (μg/mL)
Candida albicans ATCC 40006	≥1024
Candida krusei ATCC 2538	≥1024
Candida tropicalis ATCC 40042	≥1024

Table 5. Modulatory activity of the essential oil of C. limae against fungal strains.

	C. albicans ATCC 40006		C. krusei ATCC 2538		C. tropicalis ATCC 40042
Fungicides	Control	EOCL (μg/mL)	Control	EOCL (μg/mL)	Control	EOCL (μg/mL)
Amphotericin B	1024	1024	1024	1024	1024	1024
Benzoylmetronidazole	128	1024	1024	256	1024	1024
Nystatin	1024	1024	1024	1024	1024	1024

EOCL: essential oil of Croton limae.

Adams RP. 2001. Identification of essential oil components by gas mass spectroscopy. 3rd ed. Illinois: Allured Publishing Corporation.

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