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Research Article

Effects of the hydroalcoholic extract of Rosa damascena on learning and memory in male rats consuming a high-fat diet

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Pages 2065-2073 | Received 15 Mar 2016, Accepted 20 Jul 2017, Published online: 23 Aug 2017

Figures & data

Figure 1. Timeline of experiments.

Figure 1. Timeline of experiments.

Table 1. The composition of the high-fat and standard diets.

Figure 2. (A) Serum cholesterol, (B) low-density lipoprotein (LDL), (C) triglyceride (TG) and (D) high-density lipoprotein (HDL) levels in the control, high-fat diet (HFD), extract (Ext) and HFD + Ext groups. The HFD and HFD + Ext groups showed higher cholesterol and LDL levels than the control group. ***Statistically significant differences in the HFD and HFD + Ext groups compared to the control group (p < 0.001). $$$Statistically significant differences between the HFD, HFD + Ext and extract groups (p < 0.001).

Figure 2. (A) Serum cholesterol, (B) low-density lipoprotein (LDL), (C) triglyceride (TG) and (D) high-density lipoprotein (HDL) levels in the control, high-fat diet (HFD), extract (Ext) and HFD + Ext groups. The HFD and HFD + Ext groups showed higher cholesterol and LDL levels than the control group. ***Statistically significant differences in the HFD and HFD + Ext groups compared to the control group (p < 0.001). $$$Statistically significant differences between the HFD, HFD + Ext and extract groups (p < 0.001).

Figure 3. Number of trials to achieve learning in the passive avoidance test (A) step-through latency in the acquisition trial (STLa), (B) step-through latency in the retention trial (STLr) (C) time spent in the dark compartment (TDC) (D) of the passive avoidance learning test. Con = control group, Ext = extract group, HFD = High-fat diet group, HFD + Ext = High-fat diet + extract group. The HFD and extract groups showed statistically significant decreases in step-through latency in the retention test and statistically significant increases in TDC compared to the control group (**p < 0.01 and ***p < 0.001, respectively). The HFD + Ext group showed a statistically significant increase in step-through latency in the retention test compared to the HFD group (#p < 0.05; ##p < 0.01, respectively).

Figure 3. Number of trials to achieve learning in the passive avoidance test (A) step-through latency in the acquisition trial (STLa), (B) step-through latency in the retention trial (STLr) (C) time spent in the dark compartment (TDC) (D) of the passive avoidance learning test. Con = control group, Ext = extract group, HFD = High-fat diet group, HFD + Ext = High-fat diet + extract group. The HFD and extract groups showed statistically significant decreases in step-through latency in the retention test and statistically significant increases in TDC compared to the control group (**p < 0.01 and ***p < 0.001, respectively). The HFD + Ext group showed a statistically significant increase in step-through latency in the retention test compared to the HFD group (#p < 0.05; ##p < 0.01, respectively).

Figure 4. (A) Escape latency, (B) distance travelled, (C) mean swimming speed and (D) time spent in the target quadrant in the Morris water maze test. Con = control group, Ext = extract group, HFD = High-fat diet group, HFD + Ext = High-fat diet + extract group. There were no significant differences between groups.

Figure 4. (A) Escape latency, (B) distance travelled, (C) mean swimming speed and (D) time spent in the target quadrant in the Morris water maze test. Con = control group, Ext = extract group, HFD = High-fat diet group, HFD + Ext = High-fat diet + extract group. There were no significant differences between groups.