Production of eurycomanone from cell suspension culture of Eurycoma longifolia

Figures & data

Table 1. Effect of NAA on callus growth after 2 weeks of culture.

NAA (mg/L)	Fresh weight (g)	Dry weight (g)	GI	Callus morphogenesis
0.50	20.47^c	0.79^d	6.8	Light yellow and soft
0.75	21.92^e	0.88^e	7.3	Light yellow and soft
1.00	21.30^d	0.87^e	7.1	Yellow and friable
1.25	23.59^f	0.93^f	7.8	Yellow and friable
1.50	18.50^b	0.72^c	6.2	Yellow and friable
1.75	18.21^b	0.71^c	6.1	Yellow and friable
2.00	17.24^a	0.65^b	5.7	Light yellow and soft
2.25	16.79^a	0.62^a	5.6	Light yellow and soft

Different letters in a column indicate significantly different means (Duncan’s test, p < 0.05).

Figure 1. In vitro cultures of E. longifolia. (A) Callus, (B) suspension cell, (C) fresh cell biomass of 14 days-old culture after filtration and (D) fresh cell biomass after vacuum filtration.

Table 2. Effect of NAA (1.25 mg/L) and KIN on callus growth after 2 weeks of culture.

KIN (mg/L)	Fresh weight (g)	Dry weight (g)	GI	Callus morphogenesis
0.50	23.43^b	0.92^b	7.8	Light yellow and soft
0.75	30.98^cd	1.26^d	10.3	Light yellow and soft
1.00	32.34^d	1.31^d	10.7	Yellow and friable
1.25	28.80^c	1.11^c	9.6	Yellow and friable
1.50	24.65^b	0.94^b	8.2	Yellow and friable
1.75	20.49^a	0.79^a	6.8	Light yellow and soft
2.00	18.70^a	0.78^a	6.2	Light yellow and soft

Different letters in a column indicate significantly different means (Duncan’s test, p < 0.05).

Figure 2. Time course of E. longifolia cell growth.

Figure 3. HPLC chromatogram of standard eurycomanone.

Figure 4. HPLC chromatogram of eurycomanone extract from 14 days-old cell aggregate of E. longifolia.

Figure 5. HPLC chromatogram of eurycomanone extract from E. longifolia rhizome.

Figure 6. Time course of eurycomanone accumulation in E. longifolia cells.

Table 3. Effect of carbon sources on cell growth and eurycomanone accumulation after 14 days of culture.

	Glucose (g/L)			Sucrose (g/L)
Characteristics	20	30	40	20	30	40
Fresh weight (g)	17.58^a	20.30^b	12.57^c	17.62^b	17.43^b	18.00^b
Dry weight (g)	0.43^a	0.59^b	0.44^a	0.44^a	0.72^c	0.61^b
Eurycomanone (mg/g)	0.47^a	0.77^b	1.36^d	1.54d^e	1.70^e	1.11^c

Different letters in a column indicate significantly different means (Duncan’s test, p < 0.05).