Antiproliferative and cytotoxic activities of furocoumarins of Ducrosia anethifolia

Figures & data

Figure 1. Secondary metabolites isolated from Ducrosia anethifolia.

Table 1. Antiproliferative (AA) and cytotoxic activities (CA) of the furocoumarins against PAR, MDR and NIH/3T3 cells presented as IC₅₀ values.

Compounds	AA on PAR cells (µM)	AA on MDR cells (µM)	CA on PAR cells (µM)	CA on MDR cells (µM)	CA on NIH/3T3cells (µM)
Pabulenol (1)	30.47 ± 0.47	29.28 ± 0.45	51.32 ± 3.32	>100	54.09 ± 3.83
(+)-Oxypeucedanin hydrate (2)	41.96 ± 0.88	60.58 ± 2.74	>100	>100	83.55 ± 0.57
Oxypeucedanin (3)	25.98 ± 1.27	28.89 ± 0.73	40.33 ± 0.63	66.68 ± 0.00	57.18 ± 3.91
Oxypeucedanin methanolate (4)	35.88 ± 0.96	33.23 ± 0.51	56.42 ± 5.23	>100	47.16 ± 1.28
Imperatorin (6)	36.12 ± 0.91	42.24 ± 0.88	52.56 ± 4.19	>100	92.41 ± 2.80
Isogospherol (7)	46.53 ± 0.47	48.75 ± 0.28	>100	>100	54.82 ± 1.99
Heraclenin (8)	32.73 ± 2.40	46.54 ± 1.22	65.81 ± 1.00	83.94 ± 1.68	70.91 ± 4.26
Heraclenol (9)	52.31 ± 2.12	46.57 ± 0.47	>100	>100	65.78 ± 0.46
Doxorubicin	0.054 ± 0.005	0.468 ± 0.065	0.377 ± 0.02	7.152 ± 0.358	5.71 ± 0.50

Data were expressed as mean ± standard deviation (n = 3). Different letters represent significant differences (p < 0.05).

Table 2. Efflux pump inhibiting activities of furocoumarins.

Samples	Conc. μM	FSC	SSC	FL-1	FAR
PAR	–	2069	658	98.20	–
MDR	–	2152	725	1.79	–
		MDR mean		1.182	–
Tariquidar	0.02	2156	719	119	100.68
Verapamil	20	2143	740	9.69	8.20
Pabulenol (1)	2	2324	728	0.596	0.82
	20	2323	750	0.544	0.75
(+)-Oxypeucedanin hydrate (2)	2	2325	725	0.715	0.98
	20	2305	769	0.583	0.80
Oxypeucedanin (3)	2	2190	749	0.9	0.76
	20	2164	763	2.62	2.22
Oxypeucedanin methanolate (4)	2	2310	737	0.58	0.80
	20	2290	766	0.499	0.68
Imperatorin (6)	2	2165	749	0.727	0.62
	20	2161	750	0.783	0.66
Isogospherol (7)	2	2305	741	0.531	0.73
	20	2326	741	0.548	0.75
Heraclenin (8)	2	2318	723	0.942	1.29
	20	2294	764	0.57	0.78
Heraclenol (9)	2	2300	742	0.995	1.37
	20	2317	740	0.348	1.30
DMSO	2% (V/V)	2308	762	0.497	0.68
MDR	–	2301	746	0.535	–

Table 3. Checkerboard combination assay of selected compounds with doxorubicin.

Compound	Best ratio	CI at ED50	Interaction	SD (±)
Oxypeucedanin	1:50	0.85537	Slight synergism	0.07800
Heraclenin	4:100	0.88955	Slight synergism	0.06334