Inosine induces acute hyperuricaemia in rhesus monkey (Macaca mulatta) as a potential disease animal model

Figures & data

Table 1. qRT-PCR primers and parameters.

Target	Sequence (5′–3′)	Product size (bp)	Annealing temperature (°C)	Cycles
XDH/XO	TCATTTCCGCTGATGATGTTCC	106	60	40
	GCACCAATGATATGCCCAACAC
PNP	GAGACCATGGAGAACGGATACAC	120	60	40
	CAGACCTCCTAATCCAGAACCAC
URAT1	TGAGCCATGAGGGAGGAAC	119	60	40
	CCAAAGGCGAACCAGCA
OAT4	GGTGGCTGATTATTAAGGGCAAAC	122	60	40
	CTTCACGCTGGACATCAGCA
GLUT9	CCACGCTACCTGCTCTTGGA	75	60	40
	TGCTTTCCCAAGAACGTTGGA
ABCG2	TTAGCTGCAAGGAAAGATCCAAG	182	60	40
	GTCATAGTTGTTGGAAGGCCGAAAG

Figure 1. Dose-dependent effects of inosine in rhesus monkeys. Data are presented as mean ± SEM, n = 5/group. *p< 0.05; **p< 0.01 vs. control monkeys.

Figure 2. Activity of ulodesine and febuxostat in rhesus monkeys with acute HUA, as measured by the percentage change in SUA 0, 0.5, 1, 2, 4 and 8 h after administration. Data are presented as mean ± SEM, n = 5/group. *p< 0.05, **p< 0.01 vs. control monkeys. ^#p< 0.05, ^##p< 0.01 vs. 200 mg/kg inosine-treated HUA monkeys.

Figure 3. Activity of allopurinol in rhesus monkeys with acute HUA. Data are presented as mean ± SEM, n = 5/group. **p< 0.01 vs. control monkeys. ^##p< 0.01 vs. 200 mg/kg inosine-treated HUA monkeys.

Figure 4. Reverse transcription-qPCR analysis of PNP and XO in the liver tissues. Data are presented as mean ± SEM, n = 5/group. **p< 0.01 vs. control monkeys.

Figure 5. Western blotting of PNP and XO in the liver tissues. Data are presented as mean ± SEM, n = 5/group. **p< 0.01 vs. control monkeys.

Figure 6. Reverse transcription-qPCR analysis of URAT1, GLUT9, OAT4 and ABCG2 in the kidney cortex tissues. Data are presented as mean ± SEM, n = 5/group.

Figure 7. Western blotting analysis of URAT1, GLUT9, OAT4 and ABCG2 in the kidney cortex tissues. Data are presented as mean ± SEM, n = 5/group. ^#p< 0.05 vs. 200 mg/kg inosine-treated hyperuricemic monkeys. *p< 0.05 vs. control monkeys.