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Pharmaceutical Biology Volume 59, 2021 - Issue 1
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Short Communication

A pharmacokinetics–pharmacodynamics study of single-dose total glucosides of paeony capsule on reducing serum total bile acid in hepatic injury rats

Ninghua Jianga Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaView further author information
,
Bohong Zhengb Department of Pharmacy, College of Medicine, Jiaxing University, Jiaxing, ChinaView further author information
,
Yihan Fengb Department of Pharmacy, College of Medicine, Jiaxing University, Jiaxing, ChinaView further author information
,
Lei Yinb Department of Pharmacy, College of Medicine, Jiaxing University, Jiaxing, ChinaView further author information
,
Yuanrong Liub Department of Pharmacy, College of Medicine, Jiaxing University, Jiaxing, ChinaView further author information
,
Lujing Caob Department of Pharmacy, College of Medicine, Jiaxing University, Jiaxing, ChinaView further author information
,
Ning Zhengb Department of Pharmacy, College of Medicine, Jiaxing University, Jiaxing, ChinaView further author information
,
Suxiang Wuc College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, ChinaView further author information
,
Baoyue Dingb Department of Pharmacy, College of Medicine, Jiaxing University, Jiaxing, ChinaView further author information
,
Xuan Huangb Department of Pharmacy, College of Medicine, Jiaxing University, Jiaxing, ChinaView further author information
,
Jeffrey Wangd Department of Pharmaceutical Sciences, College of Pharmacy, Western University of Health Sciences, Pomona, CA, USAView further author information
&
Shuyu Zhanb Department of Pharmacy, College of Medicine, Jiaxing University, Jiaxing, ChinaCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-5973-6579View further author information
ORCID Icon show all
Pages 767-775 | Received 16 Jul 2020, Accepted 28 May 2021, Published online: 21 Jun 2021

Figures & data

Figure 1. The structure of paeoniflorin (A, C23H28O11, 480.45) and albiflorin (B, C23H28O11, 480.45).

Figure 1. The structure of paeoniflorin (A, C23H28O11, 480.45) and albiflorin (B, C23H28O11, 480.45).

Figure 2. The full chromatographic spectrum of TGP (1. Gallic acid; 2. Hydroxypaeoniflorin; 3. Catechin; 4. Albiflorin; 5. Paeoniflorin; 6. Pentagalloyl glucose; 7. Benzoic acid; 8. Benzoylpaeoniflorin; 9. Paeonol).

Figure 2. The full chromatographic spectrum of TGP (1. Gallic acid; 2. Hydroxypaeoniflorin; 3. Catechin; 4. Albiflorin; 5. Paeoniflorin; 6. Pentagalloyl glucose; 7. Benzoic acid; 8. Benzoylpaeoniflorin; 9. Paeonol).

Figure 3. MRM chromatograms of paeoniflorin, albiflorin and gentiopicroside (IS). (A) blank serum; (B) blank serum spiked with paeoniflorin, albiflorin and IS (30, 15 and 1 μg/mL respectively; (C) rat serum collected at 10 min after administration of 2.82 g/kg TGP.

Figure 3. MRM chromatograms of paeoniflorin, albiflorin and gentiopicroside (IS). (A) blank serum; (B) blank serum spiked with paeoniflorin, albiflorin and IS (30, 15 and 1 μg/mL respectively; (C) rat serum collected at 10 min after administration of 2.82 g/kg TGP.

Table 1. Precision and accuracy for the assay of paeoniflorin and albiflorin in rat serum (n = 6).

Table 2. Stability of paeoniflorin and albiflorin in rat serum and standard solutions (n = 6).

Figure 4. Mean serum concentration–time curves of paeoniflorin (A) and albiflorin (B) after intragastric administration of 2.82, 1.41 and 0.705 g/kg TGP in CCl4-induced hepatic injury rats (n = 6).

Figure 4. Mean serum concentration–time curves of paeoniflorin (A) and albiflorin (B) after intragastric administration of 2.82, 1.41 and 0.705 g/kg TGP in CCl4-induced hepatic injury rats (n = 6).

Table 3. Pharmacokinetic parameters of paeoniflorin and albiflorin in CCl4-induced hepatic injury rats after intragastric administration of 2.82, 1.41 and 0.705 g/kg TGP (n = 6).

Figure 5. Serum ALT (A), AST (B) and TBA (C) levels in rats at pre-disease modelling (Pre-M), after-disease modelling (After-M), 3 h after drug administration (Treated-3 h), 8 h after drug administration (Treated-8 h) and 20 h after drug administration (Treated-20 h). (n = 6, **p < 0.01 vs. pre-M, #p < 0.05, ##p < 0.01 vs. after-M).

Figure 5. Serum ALT (A), AST (B) and TBA (C) levels in rats at pre-disease modelling (Pre-M), after-disease modelling (After-M), 3 h after drug administration (Treated-3 h), 8 h after drug administration (Treated-8 h) and 20 h after drug administration (Treated-20 h). (n = 6, **p < 0.01 vs. pre-M, #p < 0.05, ##p < 0.01 vs. after-M).

Figure 6. Serum ALT (A), AST (B) and TBA (C) levels over time in the Control, Model, Treated-H, Treated-M and Treated-L groups after single dose administration, respectively (n = 6). Treated-H group: *p < 0.05, **p < 0.01 vs. Time = 0; Treated-M group: #p < 0.05, ##p < 0.01 vs. Time = 0; Treated-L group: Δp < 0.05, ΔΔp < 0.01 vs. Time = 0.

Figure 6. Serum ALT (A), AST (B) and TBA (C) levels over time in the Control, Model, Treated-H, Treated-M and Treated-L groups after single dose administration, respectively (n = 6). Treated-H group: *p < 0.05, **p < 0.01 vs. Time = 0; Treated-M group: #p < 0.05, ##p < 0.01 vs. Time = 0; Treated-L group: Δp < 0.05, ΔΔp < 0.01 vs. Time = 0.

Figure 7. The observed mean serum TBA levels effect (TBA-obs) and the predicted mean serum TBA levels effect (TBA-pre) vs paeoniflorin and albiflorin concentration after oral administration of 2.82 (A), 1.41 (B) and 0.705 g/kg (C) TGP in CCl4-induced acute hepatic injury rats.

Figure 7. The observed mean serum TBA levels effect (TBA-obs) and the predicted mean serum TBA levels effect (TBA-pre) vs paeoniflorin and albiflorin concentration after oral administration of 2.82 (A), 1.41 (B) and 0.705 g/kg (C) TGP in CCl4-induced acute hepatic injury rats.

Table 4. Mean pharmacodynamic parameters of paeoniflorin and albiflorin for reducing serum TBA levels effect after oral administration of 2.82, 1.41 and 0.705 g/kg TGP in CCl4-induced acute hepatic injury rats.

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