Figures & data
Table 1. Reaction conditions of CYP450 isoforms tested, including maker reactions, incubation conditions and Km used in the inhibition study.
Figure 2. Effect of physcion on the major CYP450 isoforms (A) and its concentration-dependent manner in the inhibition of CYP2C9 (B), 2D6 (C) and 3A4 (D). ****p < 0.0001 relative to the negative control. Negative control: blank treatment; physcion: treated with 100 μM physcion; positive control: treated with typical inhibitors (inhibitor types and concentrations are listed in ).
![Figure 2. Effect of physcion on the major CYP450 isoforms (A) and its concentration-dependent manner in the inhibition of CYP2C9 (B), 2D6 (C) and 3A4 (D). ****p < 0.0001 relative to the negative control. Negative control: blank treatment; physcion: treated with 100 μM physcion; positive control: treated with typical inhibitors (inhibitor types and concentrations are listed in Table 1).](/cms/asset/d6234af5-5b5b-44db-92e2-6f58a3d45052/iphb_a_2314089_f0002_b.jpg)
Figure 3. The inhibition of CYP2C9 (A) and 2D6 (B) by physcion was best fitted with the competitive inhibition model by Lineweaver–Burk plot in the presence of 0, 2, 5, 10 and 15 μM physcion and the Ki values were 3.69 and 8.66 μM, respectively.
![Figure 3. The inhibition of CYP2C9 (A) and 2D6 (B) by physcion was best fitted with the competitive inhibition model by Lineweaver–Burk plot in the presence of 0, 2, 5, 10 and 15 μM physcion and the Ki values were 3.69 and 8.66 μM, respectively.](/cms/asset/f8575b3b-cd8a-4bfc-8793-a66f4a7ec68c/iphb_a_2314089_f0003_b.jpg)
Data availability statement
The datasets used and/or analysed during this study are available from the corresponding author on reasonable request.