Intact endothelial and contractile function of coronary artery after 8 hours of heart preservation

Figures & data

Table 1. The perfusion medium used for 8-hour heart preservation.

Na+	136 mmol/L
K+	23 mmol/L
Ca2+	1.67 mmol/L
Mg2+	8.0 mmol/L
Cl-	142 mmol/L
HCO3−	25 mmol/L
PO4^2−	1.3 mmol/L
d-Glucose	6.3 mmol/L
Albumin	75 g/L
Cocaine	6 pmol/L
Noradrenaline	6 pmol/L
Adrenaline	6 pmol/L
T3	3 pmol/L
T4	2 pmol/L
Cortisol	420 pmol/L
Insulin	8 U/L
Imipenen	20 mg/L
Erythrocytes (Hct)^a
Intermittent group	15%
Continuous group	10%
95% O2 + 5% CO2^b	0.2 L/min

^a When all drugs and erythrocytes have been added and mixed and the pCO₂ has stabilized, pH is adjusted to 7.40 by means of sodium bicarbonate.

^b Administered through the oxygenator.

Table 2. Blood gases from coronary sinus during preservation.

	Intermittent perfusion		Continuous perfusion
	2 Hours	8 Hours	2 Hours	8 Hours
Hct (%)	16.9 ± 0.6	17.1 ± 0.4	10.9 ± 0.4	10.9 ± 0.5
Sat O2 (%)	96.4 ± 0.5	96.8 ± 0.3	97.5 ± 0.2	97.6 ± 0.2
pCO2 (kPa)	6.1 ± 0.1	6.37 ± 0.11	5.3 ± 0.3	6.41 ± 0.14
pH	7.26 ± 0.01	7.23 ± 0.01	7.30 ± 0.02	7.22 ± 0.01
HCO3 (mmol/L)	23.9 ± 0.5	23.1 ± 0.4	24.0 ± 0.3	24.3 ± 0.5
ABE (mmol/L)	−0.6 ± 0.5	−1.7 ± 0.5	−0.5 ± 0.3	−0.2 ± 0.6
Glucose (mmol/L)	7.9 ± 0.5	7.9 ± 0.5	7.1 ± 0.2	7.2 ± 0.2
Lactate (mmol/L)	1.0 ± 0.3	1.9 ± 0.3	0.3 ± 0.1	0.2 ± 0.1

Table 3. Coronary smooth muscle contraction and EDR and endothelium-independent relaxation.

	Fresh control	I	C
Contraction with 127 mmol/L potassium-Krebs (mN)	53.01 ± 3.18	52.09 ± 3.74	57.37 ± 3.21
Contraction with 3 × 10^−8 mol/L of U-46619 (mN)	36.30 ± 3.11	41.04 ± 1.42	43.57 ± 2.55
Max EDR with Substance P (%)	93.3 ± 1.3	95.7 ± 1.5	95.8 ± 0.5
Negative logarithm of the concentration of Substance P giving half maximal relaxation	6.21 ± 0.11	6.31 ± 0.06	6.44 ± 0.07
Max endothelium-independent relaxation with 10^−4 mol/L papaverine (%)	104.2 ± 0.3	103.3 ± 0.7	101.7 ± 0.2

Figure 1. EDR elicited by Substance P on a thromboxane A₂-induced stable contraction. Results are given as mean ± SEM, n = 8.