Serum microRNA-125b-5p expression in patients with dilated cardiomyopathy combined with heart failure and its effect on myocardial fibrosis

Figures & data

Table 1. Primer sequences for genes in RT-qPCR (rat).

Gene	Primer sequences (5’-3’)
miR-125b-5p	F: 5′-TCCCTGAGACCCTAACTTGTGA-3′
	R: 5′-AGTCTCAGGGTCCGAGGTATTC-3′
U6	F: 5′-GCTTCGGCAGCACATATACTAAAAT-3′
	R: 5′-CGCTTCACGAATTTGCGTGTCAT-3′
miR-125b-5p	F: 5′-TCCCTGAGACCCTAACTTGTGA-3′
	R: 5′-GCGAGCACAGAATTAATACGACT-3′
TGF-1β	F: 5′-GACCGCAACAACGCAATCT-3′
	R: 5′-GACAGCCACTCAGGCGTATC-3′
Collagen I	F: 5′-TTACCCTGGCAACATTGGTC-3′
	R: 5′-CCTTGTCACCTCGAATACCTTG-3′
α-SMA	F: 5′-ACCATCGGGAATGAACGCTT-3′
	R: 5′-CTGTCAGCAATGCCTGGGTA-3′
GAPDH	F: 5′-CTGGAGAAACCTGCCAAGTATG-3′
	R: 5′-GGTGGAAGAATGGGAGTTGCT-3′

Figure 1. Model establishment and detection process diagram.

Figure 2. The flowchart of detection indicators and the mechanism diagram of this study.

Table 2. Comparison of the general data between the two groups.

Item	Control group (n = 49)	Case group (n = 49)	p
Age (years)	60.98 ± 6.63	59.27 ± 6.67	0.205
Gender [case (%)]			0.838
Male	29 (59.18)	27 (55.10)
Female	20 (40.82)	22 (44.90)
Body mass index (kg/m^2)	23.94 ± 5.42	24.71 ± 5.47	0.486
Course of disease (year)	–	5.24 ± 1.56	–
Smoking history [case (%)]	24 (48.98)	28 (57.14)	0.544
Drinking history [case (%)]	21 (42.86)	23 (46.94)	0.839
NYHA classification [case (%)]			–
II	–	15 (30.61)
III	–	22 (44.90)
IV	–	12 (24.49)

Table 3. Comparison of cardiac function between the two groups.

Group	miR-125b-5p	NT-pro-BNP (pg/ml)	LVEDD (mm)	LVESD (mm)	LVEF (%)	LVFS (%)
Control group (n = 49)	1.00 ± 0.20	210.27 ± 12.36	41.96 ± 4.35	34.77 ± 4.99	63.36 ± 4.28	36.48 ± 4.05
Case group (n = 49)	0.69 ± 0.16	683.32 ± 115.29	61.71 ± 6.34	51.36 ± 4.67	32.73 ± 4.58	17.69 ± 3.94
P	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

Figure 3. Clinical diagnostic value of miR-125b-5p level in DCM with HF by ROC curve analysis.

Table 4. Analysis of the correlation between miR-125b-5p and each indicator in patients.

	miR-125b-5p
	R	P
LVEDD	−0.685	<0.001
LVESD	−0.726	<0.001
LVEF	0.458	<0.001
LVFS	0.505	<0.001
NYHA cardiac function classification	−0.491	<0.001
NT-proBNP	−0.472	<0.001

Figure 4. Cardiac function of rats in each group. A-D: Echocardiographic detection of LVEDD, LVESD, LVEF, and LVFS in rats. * p < 0.05 vs. CON group; # p < 0.05 vs. DOX group; $ p < 0.05 vs. DOX + NC agomir group.

Figure 5. Overexpression of miR-125b-5p effectively improves myocardial fibrosis in DCM rats. A. RT-qPCR was utilized to detect miR-125b-5p expression in rats of each group. B. HE and masson stainings were employed to observe the histologic changes of myocardial pathology in rats. C-E. ELISA was adopted to measure the levels of TNF-α, hs-CRP, and NT-proBNP in rats of each group. F-H. RT-qPCR was implemented to test the expression of TGF-1β, collagenI, and α-SMA factors in rats of each group. I. TUNEL staining was performed to examine apoptotic cardiomyocytes in rats of each group. * p < 0.05 vs. CON group; # p < 0.05 vs. DOX group; $ p < 0.05 vs. DOX + NC agomir group.

Data availability statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.