Evaluation of F1 and BC1 hybrids of Lilium lancifolium × Asiatic hybrid ‘Chianti’ by morphological analysis and fluorescence in situ hybridization

ABSTRACT

Lilium lancifolium Thunb. exhibits wide genetic diversity and numerous genetic traits within progeny populations. Parent morphology affects the distribution and assortment of progeny phenotypes. In this study, morphological analysis and fluorescence in situ hybridization (FISH) were conducted for cross progeny (F1 and BC1). Results showed that F1-1 hybrids had greater plant height than the parents; however, backcross progeny plant height (group 1) was low. Leaf number in both groups was considerably low in F1 hybrid, although high in backcrossed plants. Flower number and days to flower opening were intermediate to those of the parents for F1 hybrids, yet low in BC1 progeny. In group 1, backcross progeny showed small bulb length; however, group 2 progeny showed large bulb length. Positive and negative correlations between the phenotypic traits of parents and progeny confirmed significant variations. According to FISH results, F1-1 and F1-2 hybrids distinctly exhibited nine and eight 45S rDNA loci which were same in position with 45S loci of the parents. In backcross progeny, eight 45S rDNA signals were detected in four BC1 progeny of group 1, while 10 signals were observed in all group 2 progeny, same with the L. lancifolium karyotype. Morphological analysis and FISH helped in scrutinising progeny to obtain hybrids with desirable characteristics.

KEYWORDS:

• phenotype
• chromosome
• 45S rDNA
• 5S rDNA
• progeny
• feature

Acknowledgment

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Project No. NRF-2016R1D1A1B04932913) and the Regional Subgenebank Support Program of Rural Development Administration, Republic of Korea (No.PJ012891).

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the National Research Foundation of Korea (NRF) [NRF-2016R1D1A1B04932913]; regional Subgenebank Support Programme Rural Development Administration, Republic of Korea [PJ012177].