https://orcid.org/0000-0002-8971-0775
Figures & data
Figure 1. Illustration of workflow in Real-time PCR, CRISPR, and TALEN. In Real-time PCR, the trained operators require specific kits and facilities, but in CRISPR, a fragment of interest region of RNA target in isothermal condition and by reverse transcription-recombinase polymerase amplification (RT-RPA), is amplified to DNA and then by T7 transcription converted to RNA. The attachment of Cas13a–CRISPR RNA to amplified target RNAs stimulates the collateral activity of Cas13a, which then cleaves reporters. Separated RNA reporters should be captured on a colorimetric lateral-flow strip (biotin–fluorescein RNA reporter) or visualized by fluorescence signal (fluorescent reporter). A similar procedure is performed in TALEN but with more efficiency.
