In vitro effects of some drugs on catalase purified from human skin

Figures & data

Table I.  Scheme for Purification of catalase from human skin.

Purification step	Activity (EU/ml)	Total volume (ml)	Protein (mg/ml)	Total protein (mg)	Total activity (EU)	Specific activity (EU/mg)	Yield (%)	Purification factor
Homogenate	76.76	72	62.7	4514.4	5526.7	1.22	100	1
Ammonium sulfate precipitation (20–50)%	137.46	22	14.1	310.2	3024.1	9.74	54.7	7.98
DEAE-Sephadex A50 ion exchange chromatography	149	16	0.11	1.76	2384	1354.5	43.13	1110.2

Figure 1 SDS-PAGE bands of G6PD Lane 1: Human skin CAT. Lane 2: Standards: Rabbit myosin (205,000), E.Coli (-galactosidase (116,000), rabbit phosphorylase B (97,400), bovine albumin (66,000), chicken ovalbumin (45,000), and bovine carbonic anhydrase (29,000)).

Figure 2 % Activity vs drug concentration regression analysis graphs for human skin CAT in the presence of 5 different concentrations of, (A) piroxicam, (B) Retoprofen, (C) diclofenac, (D) sulfamethoxazole, (E) nidazole.

Table II.  I₅₀ values for test drugs

Drug	I50 (mM)
Piroxicam	0.414
Ketoprofen	1.29
Diclofenac Sodium	1.8
Sulfamethoxazole	3.83
Nidazole	8.64

obtained from regression analysis graphs in the presence of 5 different drugs concentrations.