Figures & data
Table I. Concentrations and proportional factors for ethanol.
Figure 1 Cholinesterase activities vs. substrate concentrations (pS curves) for electric eel and insect AChE, and horse BChE (as indicated) in the absence and presence of various concentrations of methanol and ethanol (as indicated).
![Figure 1 Cholinesterase activities vs. substrate concentrations (pS curves) for electric eel and insect AChE, and horse BChE (as indicated) in the absence and presence of various concentrations of methanol and ethanol (as indicated).](/cms/asset/9d707bb7-bc8b-4e26-b53b-34338f698b92/ienz_a_214316_f0001_b.gif)
Table II. Kinetic constants for the cholinesterases in the presence of substrate, methanol and ethanol according to the scheme given in Appendix.
Figure 2 Time-courses of acetylcholinesterase inhibition by eserine. A. Electric eel AChE. B. Drosophila melanogaster AChE.
![Figure 2 Time-courses of acetylcholinesterase inhibition by eserine. A. Electric eel AChE. B. Drosophila melanogaster AChE.](/cms/asset/013e6703-97ad-479a-b5b9-471d93b047e8/ienz_a_214316_f0002_b.gif)
Figure 3 Time-courses of electric eel AChE inhibition by 0.1 mM DFP in the absence and presence of 10% ethanol. Inset: semilogarithmic plot.
![Figure 3 Time-courses of electric eel AChE inhibition by 0.1 mM DFP in the absence and presence of 10% ethanol. Inset: semilogarithmic plot.](/cms/asset/2310542e-d848-4f52-a727-edf907876bdc/ienz_a_214316_f0003_b.gif)
Figure 4 Gas chromatograms of various mixtures of ethanol, acetylcholine, cholinesterase, ethyl acetate and sulfuric acid: A) the remaining ethanol from the previous run + ethyl acetate (20%); B) sodium acetate (2 mM) + ethanol (20%)+ sulfuric acid (2 mM); C) ethanol (20%)+ acetylcholine (50 mM); D) ethanol (20%)+ acetylcholine (50 mM) + cholineterase (∼1 nM); E) ethanol (20%)+ acetylcholine (50 mM), after a 2-h incubation; F) ethanol (20%)+ acetylcholine (50 mM) + cholineterase (∼1 nM), after a 2-h incubation.
![Figure 4 Gas chromatograms of various mixtures of ethanol, acetylcholine, cholinesterase, ethyl acetate and sulfuric acid: A) the remaining ethanol from the previous run + ethyl acetate (20%); B) sodium acetate (2 mM) + ethanol (20%)+ sulfuric acid (2 mM); C) ethanol (20%)+ acetylcholine (50 mM); D) ethanol (20%)+ acetylcholine (50 mM) + cholineterase (∼1 nM); E) ethanol (20%)+ acetylcholine (50 mM), after a 2-h incubation; F) ethanol (20%)+ acetylcholine (50 mM) + cholineterase (∼1 nM), after a 2-h incubation.](/cms/asset/782184dc-8084-45c9-b088-587ad7726928/ienz_a_214316_f0004_b.gif)