Figures & data
Figure 1 Lineweaver-Burk double reciprocal plot of initial velocity against GSSG as varied substrate and ZnSO4 (0.05–1 mM) as inhibitor at a fixed NADPH (0.1 mM) concentration. *0.1 mM NADPH (constant); □ 0.05 mM ZnSO4 ;○ 0.1 mM ZnSO4; Δ 0.5 mM ZnSO4; ⋄1 mM ZnSO4.
![Figure 1 Lineweaver-Burk double reciprocal plot of initial velocity against GSSG as varied substrate and ZnSO4 (0.05–1 mM) as inhibitor at a fixed NADPH (0.1 mM) concentration. *0.1 mM NADPH (constant); □ 0.05 mM ZnSO4 ;○ 0.1 mM ZnSO4; Δ 0.5 mM ZnSO4; ⋄1 mM ZnSO4.](/cms/asset/bce8a561-bb28-45f9-b7a8-41685ecdbcd6/ienz_a_216143_f0001_b.gif)
Figure 2 Lineweaver-Burk double reciprocal plot of initial velocity against NADPH as varied substrate and ZnSO4 (0.05–1 mM) as inhibitor at different fixed GSSG (0.7 mM) concentrations. ⋄ 0.7 mM GSSG (constant); □ 0.05 mM ZnSO4;Δ 0.1 mM ZnSO4;*0.5 mM ZnSO4;○ 1 mM ZnSO4.
![Figure 2 Lineweaver-Burk double reciprocal plot of initial velocity against NADPH as varied substrate and ZnSO4 (0.05–1 mM) as inhibitor at different fixed GSSG (0.7 mM) concentrations. ⋄ 0.7 mM GSSG (constant); □ 0.05 mM ZnSO4;Δ 0.1 mM ZnSO4;*0.5 mM ZnSO4;○ 1 mM ZnSO4.](/cms/asset/2ac66891-f542-4d1d-89f5-53684669053b/ienz_a_216143_f0002_b.gif)
Figure 3 Lineweaver-Burk double reciprocal plot of initial velocity against GSSG as varied substrate and CaCl2 (0.8–1.6 mM) as inhibitor at a fixed NADPH (0.1 mM) concentrations. *0.1 mM NADPH (constant); □ 0.8 mM CaCl2; ○ 1 mm CaCl2; Δ 1.2 mM CaCl2; ⋄1.6 mM CaCl2.
![Figure 3 Lineweaver-Burk double reciprocal plot of initial velocity against GSSG as varied substrate and CaCl2 (0.8–1.6 mM) as inhibitor at a fixed NADPH (0.1 mM) concentrations. *0.1 mM NADPH (constant); □ 0.8 mM CaCl2; ○ 1 mm CaCl2; Δ 1.2 mM CaCl2; ⋄1.6 mM CaCl2.](/cms/asset/7666f6f9-9c2c-487c-924f-ef231c54d68f/ienz_a_216143_f0003_b.gif)
Figure 4 Lineweaver-Burk double reciprocal plot of initial velocity against NADPH as varied substrate and CaCl2 (0.8–1.6 mM) as inhibitor at different fixed GSSG (0.7 mM) concentrations. ⋄ 0.7 mM GSSG (constant); □ 0.8 mM CaCl2; Δ 1 mm CaCl2; *1.2 mM CaCl2; ○ 1.6 mM CaCl2.
![Figure 4 Lineweaver-Burk double reciprocal plot of initial velocity against NADPH as varied substrate and CaCl2 (0.8–1.6 mM) as inhibitor at different fixed GSSG (0.7 mM) concentrations. ⋄ 0.7 mM GSSG (constant); □ 0.8 mM CaCl2; Δ 1 mm CaCl2; *1.2 mM CaCl2; ○ 1.6 mM CaCl2.](/cms/asset/6944825c-1a75-462e-bc38-be202d7679fd/ienz_a_216143_f0004_b.gif)