Isolation and characterization of a novel adenosine deaminase inhibitor, IADA-7, from Bacillus sp. J-89

Figures & data

Figure 1.  Purification of an ADA inhibitor, IADA-7, from Bacillus sp. J-89.

Figure 2.  Characterization of chemical structure of IADA-7. The chemical structure of IADA-7 was analyzed sequentially using an IR spectrum (A), ¹H-NMR spectrum (B), and ¹³C-¹H NMR spectrum (C).

Figure 3.  Deduced structure of IADA-7.

Figure 4.  Cytotoxic effect of IADA-7 on human Jurkat T cells (- - -○- - -) and human bladder carcinoma J 82 cells (•). Both cell lines were treated with the indicated concentration of IADA-7, and its cytotoxic effect was assessed via an MTT assay, as described in the Materials and Methods.

Figure 5.  Effect of purified IADA-7 on Jurkat T cells analysed by flow cytometry. Cells were treated with IADA-7 (15 μg/mL) for 0–48 h and assessed by propidium iodide (PI) staining, as described in Materials and Methods. Grey line represents control cells, and black line represents 24 h, 48 h sample cells, respectively. Apo: apoptotic percentage.