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Research Article

Superoxide radicals scavenging and xanthine oxidase inhibitory activity of magnesium lithospermate B from Salvia miltiorrhiza

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Pages 663-668 | Received 02 Mar 2008, Accepted 19 May 2008, Published online: 20 Oct 2008

Figures & data

Figure 1. The structure of Magnesium lithopermate B.

Figure 1.  The structure of Magnesium lithopermate B.

Figure 2. Effect of MLB on (A) NBT reduction induced by superoxide radicals generated in an NADH/PMS system (B) NBT reduction induced by superoxide radicals generated in xanthine/xanthine oxidase system and (C) xanthine oxidase activity. Values show mean ± S.E.M from four experiments performed in triplicate.

Figure 2.  Effect of MLB on (A) NBT reduction induced by superoxide radicals generated in an NADH/PMS system (B) NBT reduction induced by superoxide radicals generated in xanthine/xanthine oxidase system and (C) xanthine oxidase activity. Values show mean ± S.E.M from four experiments performed in triplicate.

Figure 3. Lineweaver-Burk plots of the inhibition of xanthine oxidase by MLB. Enzyme activity was measured using 60, 120, 180, 240 and 300 μM of xanthine as a substrate. The data represented the mean ± SEM from four experiments performed in triplicate.

Figure 3.  Lineweaver-Burk plots of the inhibition of xanthine oxidase by MLB. Enzyme activity was measured using 60, 120, 180, 240 and 300 μM of xanthine as a substrate. The data represented the mean ± SEM from four experiments performed in triplicate.

Figure 4. Effects of MLB on serum urate level of oxonate hyperuricemia in mice. Values were mean ± S.E.M (n = 10). ** P < 0.01 when compared with the hyperuricemic control. All drugs were given orally except potassium oxonate which was injected i.p.

Figure 4.  Effects of MLB on serum urate level of oxonate hyperuricemia in mice. Values were mean ± S.E.M (n = 10). ** P < 0.01 when compared with the hyperuricemic control. All drugs were given orally except potassium oxonate which was injected i.p.

Figure 5. Protective effect of MLB on HL-60 cells apoptosis detected by Annexin V- PI. File001: Normal control cell; File002: Cell apoptosis induced by superoxide radicals without MLB; apoptosis rate is 62.16 ± 5.84%; File003: Cell apoptosis induced by superoxide radicals pretreated with 3 μg/mL MLB; apoptosis rate is 37.42 ± 4.32%; File004: Cell apoptosis induced by superoxide radicals pretreated with 6 μg/mL MLB; apoptosis rate is 15.44 ± 1.87%.

Figure 5.  Protective effect of MLB on HL-60 cells apoptosis detected by Annexin V- PI. File001: Normal control cell; File002: Cell apoptosis induced by superoxide radicals without MLB; apoptosis rate is 62.16 ± 5.84%; File003: Cell apoptosis induced by superoxide radicals pretreated with 3 μg/mL MLB; apoptosis rate is 37.42 ± 4.32%; File004: Cell apoptosis induced by superoxide radicals pretreated with 6 μg/mL MLB; apoptosis rate is 15.44 ± 1.87%.

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