Figures & data
Figure 2. Effect of MLB on (A) NBT reduction induced by superoxide radicals generated in an NADH/PMS system (B) NBT reduction induced by superoxide radicals generated in xanthine/xanthine oxidase system and (C) xanthine oxidase activity. Values show mean ± S.E.M from four experiments performed in triplicate.
![Figure 2. Effect of MLB on (A) NBT reduction induced by superoxide radicals generated in an NADH/PMS system (B) NBT reduction induced by superoxide radicals generated in xanthine/xanthine oxidase system and (C) xanthine oxidase activity. Values show mean ± S.E.M from four experiments performed in triplicate.](/cms/asset/430ad3f7-a822-47ea-b381-b51c1d7d7fc9/ienz_a_332549_f0002_b.gif)
Figure 3. Lineweaver-Burk plots of the inhibition of xanthine oxidase by MLB. Enzyme activity was measured using 60, 120, 180, 240 and 300 μM of xanthine as a substrate. The data represented the mean ± SEM from four experiments performed in triplicate.
![Figure 3. Lineweaver-Burk plots of the inhibition of xanthine oxidase by MLB. Enzyme activity was measured using 60, 120, 180, 240 and 300 μM of xanthine as a substrate. The data represented the mean ± SEM from four experiments performed in triplicate.](/cms/asset/99416a5e-3638-412b-a5b1-b5ed79f7d493/ienz_a_332549_f0003_b.gif)
Figure 4. Effects of MLB on serum urate level of oxonate hyperuricemia in mice. Values were mean ± S.E.M (n = 10). ** P < 0.01 when compared with the hyperuricemic control. All drugs were given orally except potassium oxonate which was injected i.p.
![Figure 4. Effects of MLB on serum urate level of oxonate hyperuricemia in mice. Values were mean ± S.E.M (n = 10). ** P < 0.01 when compared with the hyperuricemic control. All drugs were given orally except potassium oxonate which was injected i.p.](/cms/asset/7c47aa3e-8fed-4a8f-963b-97b2d1bdbf3a/ienz_a_332549_f0004_b.gif)
Figure 5. Protective effect of MLB on HL-60 cells apoptosis detected by Annexin V- PI. File001: Normal control cell; File002: Cell apoptosis induced by superoxide radicals without MLB; apoptosis rate is 62.16 ± 5.84%; File003: Cell apoptosis induced by superoxide radicals pretreated with 3 μg/mL MLB; apoptosis rate is 37.42 ± 4.32%; File004: Cell apoptosis induced by superoxide radicals pretreated with 6 μg/mL MLB; apoptosis rate is 15.44 ± 1.87%.
![Figure 5. Protective effect of MLB on HL-60 cells apoptosis detected by Annexin V- PI. File001: Normal control cell; File002: Cell apoptosis induced by superoxide radicals without MLB; apoptosis rate is 62.16 ± 5.84%; File003: Cell apoptosis induced by superoxide radicals pretreated with 3 μg/mL MLB; apoptosis rate is 37.42 ± 4.32%; File004: Cell apoptosis induced by superoxide radicals pretreated with 6 μg/mL MLB; apoptosis rate is 15.44 ± 1.87%.](/cms/asset/07c1e53b-48cb-43c5-9349-46431cb21d18/ienz_a_332549_f0005_b.gif)