Figures & data
Figure 1. A: IAP activity before and after EGTA addition. B: IAP activity with different calcium concentrations obtained after calcium addition to IAP that was previously treated with EGTA 50 mmole/L. C: Western blot of IAP before (lane 1) and after EGTA addition (lane 2) and after 50 mmole/L calcium addition (lane 3). Data are expressed as mean ± SD (n = 4).
![Figure 1. A: IAP activity before and after EGTA addition. B: IAP activity with different calcium concentrations obtained after calcium addition to IAP that was previously treated with EGTA 50 mmole/L. C: Western blot of IAP before (lane 1) and after EGTA addition (lane 2) and after 50 mmole/L calcium addition (lane 3). Data are expressed as mean ± SD (n = 4).](/cms/asset/80ed4210-1298-426c-8f86-7d21c6819026/ienz_a_333485_f0001_b.gif)
Table I. Molecular mass and isoelectric point obtained by 2D-electrophoresis for the dimer and the aggregated form with and without calcium addition.
Figure 2. A: IAP activity before and after calcium addition. B: IAP activity as a function of calcium concentration. Calcium concentrations of the horizontal axis are obtained after different additions of EGTA to the solution that started with 50 mmole Ca/L. C: Western blot of IAP before (lane 1) and after Ca2 + addition (lane 2) and after EGTA addition (lane 3) to obtain 0 mmole/L free calcium. Data are expressed as mean ± SD (n = 4).
![Figure 2. A: IAP activity before and after calcium addition. B: IAP activity as a function of calcium concentration. Calcium concentrations of the horizontal axis are obtained after different additions of EGTA to the solution that started with 50 mmole Ca/L. C: Western blot of IAP before (lane 1) and after Ca2 + addition (lane 2) and after EGTA addition (lane 3) to obtain 0 mmole/L free calcium. Data are expressed as mean ± SD (n = 4).](/cms/asset/408d557e-8c1f-499b-8de8-b870eec184c1/ienz_a_333485_f0002_b.gif)