Figures & data
Figure 1. Scheme of the cell-based multi-substrate assay for the identification of class-selective HDAC inhibitors.
Figure 2. UHPLC-ESI-MS/MS detection of acetylated and deacetylated substrates with the cell-based multi-substrate assay. (A) Chemical structures of MAL, MOCPAC, BATCP; (B) chromatograms of MAL, MOCPAC, BATCP and their deacetylated products (designated as dMAL, dMOCPAC and dBATCP, respectively) in control and TSA-treated cells (31.25 nM) and (C) HDAC inhibitory activity by TSA at various concentrations with the MAL substrate.
Figure 3. HDAC inhibitors used for assay development.
Table 1. IC50 values calculated for HDAC selective and pan-inhibitors in a cell-based assay with multi-substrate and single-substrate approaches.
Table 2. IC50 values calculated for HDAC selective and pan-inhibitors using a mixture of HDAC isoforms in both multi-substrate and single-substrate approaches.
Supplemental material
