Microextraction by packed sorbent and HPLC–PDA quantification of multiple anti-inflammatory drugs and fluoroquinolones in human plasma and urine

Figures & data

Figure 1. Chemical structures of NSAIDs, FLQs and internal standards.

Table 1. Chromatographic gradient elution.

Time (min)	% A*	% B†
0	86	14
9	86	14
15	50	50
30	50	50
30	86	14
40	86	14

*Phosphate buffer, pH 2.5, 30 mM, 1% v/v TEA.

† AcN, 1% v/v TEA; temperature 25 °C ±1 °C.

Figure 2. Chromatograms of different samples after MEPS extraction. Top side, plasma MEPS–HPLC–PDA chromatogram: (a) double blank, (b) blank spiked with internal standards and (c) blank spiked with internal standards and 1 μg/mL of all analytes. Bottom side, urine MEPS–HPLC–PDA chromatogram: (a) double blank, (b) blank spiked with internal standards and (c) blank spiked with internal standards and 1 μg/mL of all analytes. About 20 μL of samples injected at 285 nm. Key legend: levofloxacin (1), ciprofloxacin (2), enrofloxacin (IS1), ulifloxacin (3), sarafloxacin (IS2), moxifloxacin (4), furprofen (5), indoprofen (6), ketoprofen (7), fenbufen (8), flurbiprofen (9), indomethacin (10) and ibuprofen (11).

Table 2. Quantitative analysis of NSAIDs contained in commercial medications after the oral administration in human volunteers.

	Volume (mL)	Time (hours)	Concentration (μg/mL)	Total concentration (mg)	Concentration (μg/mL)	Total Concentration (mg)
Plasma
Oki® 80 mg	4400*	5	4.02	17.7	3.95†	17.4†
Momentact® 400 mg	4400*	5	9.56	42.1	8.73†	38.4†
Urine
Oki® 80 mg	400*	5	0.93	0.37	0.89†	0.36†
Momentact® 400 mg	200*	5	1.19	0.24	1.31†	0.26†

*These values were obtained from literature27.

† These values were previously published in literature17.

The other data are experimental results, which were compared to data previously published to control the performance of the HPLC–PDA and MEPS extraction.

Cameron JR, Skofronick JG, Roderick MG. Physics of the body. 2nd ed. Madison, WI: Medical Physics Publishing; 1999

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Locatelli M, Ferrone V, Cifelli R, et al Microextraction by packed sorbent and high performance liquid chromatography determination of seven non-steroidal anti-inflammatory drugs in human plasma and urine. J Chromatogr A 2014;1367:1–8

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