Screening assay for inhibitors of a recombinant Streptococcus pneumoniae UDP-glucose pyrophosphorylase

Figures & data

Table 1. Purification of UDP-Glc pyrophosphorylase.

Product	Volume (ml)	Amount of protein (mg)	Total activity (U)*	Specific activity (U/mg)	Yield (%)	Purification (fold)
Crude extract	10	66	23.8 × 10^7	3.6 × 10^6		1
GalU	1	8.8	4.4 × 10^7	5.05 × 10^6	18.69	1.40

* Enzymatic activity was assayed using the standard method described in “Materials and methods” section.

Figure 1. Percentage of inhibition activity of GalU with different nucleosides analogs.

Figure 2. Structure of the nucleoside analogs and the natural substrate of UDP-glucose pyrophosphorylase, UTP.