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Research Article

Purification and biochemical characterization of a novel thermostable serine alkaline protease from Aeribacillus pallidus C10: a potential additive for detergents

, , , ORCID Icon, ORCID Icon & ORCID Icon
Pages 468-477 | Received 08 Aug 2016, Accepted 18 Oct 2016, Published online: 18 Jan 2017

Figures & data

Table 1. Summary of the alkaline protease from A. pallidus C10 purification procedure.

Figure 1. Neighbor-joining phylogenetic tree on the basis of 16S rRNA gene sequence data of the thermophilic bacteria. Alicyclobacillus acidiphilus DSM 14558 was used as out-group. Bootstrap values based on 1000 replications are listed as percentages at branching points. Only bootstrap values >50% are shown at nodes. The scale bar represented 1% divergence.

Figure 1. Neighbor-joining phylogenetic tree on the basis of 16S rRNA gene sequence data of the thermophilic bacteria. Alicyclobacillus acidiphilus DSM 14558 was used as out-group. Bootstrap values based on 1000 replications are listed as percentages at branching points. Only bootstrap values >50% are shown at nodes. The scale bar represented 1% divergence.

Figure 2. SDS-PAGE of the purified protease from A. pallidus C10. (A) Lane 1: Standard protein molecular mass markers, lane 2: (NH4)2SO4 precipitated proteins, lane 3–4: Purified protease from DE52 anion exchange chromatography, (B) Lane 1–2: Purified enzyme from Probond affinity chromatography, lane 3: Zymography of the purified enzyme.

Figure 2. SDS-PAGE of the purified protease from A. pallidus C10. (A) Lane 1: Standard protein molecular mass markers, lane 2: (NH4)2SO4 precipitated proteins, lane 3–4: Purified protease from DE52 anion exchange chromatography, (B) Lane 1–2: Purified enzyme from Probond affinity chromatography, lane 3: Zymography of the purified enzyme.

Figure 3. Effect of pH on activity (A) and stability (B) of the purified protease from A. pallidus C10.

Figure 3. Effect of pH on activity (A) and stability (B) of the purified protease from A. pallidus C10.

Figure 4. Effect of temperature on activity (A) and stability (B) of the purified protease from A. pallidus C10.

Figure 4. Effect of temperature on activity (A) and stability (B) of the purified protease from A. pallidus C10.

Table 2. The effect of inhibitors on A. pallidus C10 alkaline protease activity.

Table 3. The effect of metal ions on A. pallidus C10 alkaline protease activity.

Table 4. The effect of organic solvents on A. pallidus C10 alkaline protease activity.

Table 5. The effect of various surfactants and the oxidant agent on A. pallidus C10 alkaline protease activity.

Figure 5. Stability of the alkaline protease from A. pallidus C10 in the presence of various commercial detergents.

Figure 5. Stability of the alkaline protease from A. pallidus C10 in the presence of various commercial detergents.