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Journal of Enzyme Inhibition and Medicinal Chemistry Volume 32, 2017 - Issue 1
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Research Article

Quantitative assessment of specific carbonic anhydrase inhibitors effect on hypoxic cells using electrical impedance assays

Luciana StanicaInternational Centre of Biodynamics, Bucharest, Romania; ;Faculty of Biology, University of Bucharest, Bucharest, Romania; View further author information
,
Mihaela GheorghiuInternational Centre of Biodynamics, Bucharest, Romania; View further author information
,
Miruna StanDepartment of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, Bucharest, Romania; View further author information
,
Cristina PolonschiiInternational Centre of Biodynamics, Bucharest, Romania; View further author information
,
Sorin DavidInternational Centre of Biodynamics, Bucharest, Romania; View further author information
,
Dumitru BratuInternational Centre of Biodynamics, Bucharest, Romania; View further author information
,
Anca DinischiotuDepartment of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, Bucharest, Romania; View further author information
,
Claudiu T. SupuranNeurofarba Department, Sezione di Scienze Farmaceutiche, Università degli Studi di Firenze, Sesto Fiorentino (Firenze), ItalyView further author information
&
Eugen GheorghiuInternational Centre of Biodynamics, Bucharest, Romania; ;Faculty of Biology, University of Bucharest, Bucharest, Romania; Correspondence[email protected]
View further author information
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Pages 1079-1090 | Received 20 Jun 2017, Accepted 11 Jul 2017, Published online: 07 Aug 2017

Figures & data

Figure 1. (a) The normalised impedance values (imaginary part at 10 kHz) increase as cells adhere and spread on the electrodes until a monolayer is formed. Data are represented as mean ± SD for n = 7. The electrode set-up and the simplified equivalent circuit for assessing EIS data on cell monolayers are presented in the inset. (b) The response of cells to hypoxic conditions, two subsequent hypoxia phases- impedance data (the imaginary part at 10 kHz). Data are represented as mean ± SD for n = 6. Inset CA IX overexpression highlighted through fluorescent CAI #1 binding.

Figure 1. (a) The normalised impedance values (imaginary part at 10 kHz) increase as cells adhere and spread on the electrodes until a monolayer is formed. Data are represented as mean ± SD for n = 7. The electrode set-up and the simplified equivalent circuit for assessing EIS data on cell monolayers are presented in the inset. (b) The response of cells to hypoxic conditions, two subsequent hypoxia phases- impedance data (the imaginary part at 10 kHz). Data are represented as mean ± SD for n = 6. Inset CA IX overexpression highlighted through fluorescent CAI #1 binding.
Supplemental material

IENZ_1355306_Supplemental_Material.pdf

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