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Research Paper

The intensification of anticancer activity of LFM-A13 by erythropoietin as a possible option for inhibition of breast cancer

, , , , , , ORCID Icon, , , , , , & show all
Pages 1697-1711 | Received 29 Apr 2020, Accepted 28 Aug 2020, Published online: 10 Sep 2020

Figures & data

Figure 2. Representative dot-plots presenting the loss of mitochondrial membrane potential (MMP) in MCF-7 (A), and MDA-MB-231 (B) cells incubated with Epo (Epo100, 100 IU/ml) and LFM-A13 (LFM100, 100 μM) for 48 h (mean ± SD; n = 3). Cells with normal MMT are shown on the left side of the plots, cells with decreased MMT on the right side of the plots.

Figure 2. Representative dot-plots presenting the loss of mitochondrial membrane potential (MMP) in MCF-7 (A), and MDA-MB-231 (B) cells incubated with Epo (Epo100, 100 IU/ml) and LFM-A13 (LFM100, 100 μM) for 48 h (mean ± SD; n = 3). Cells with normal MMT are shown on the left side of the plots, cells with decreased MMT on the right side of the plots.

Figure 5. Representative examples of immunohistochemistry for BTK expression in breast cancer cells.

Figure 5. Representative examples of immunohistochemistry for BTK expression in breast cancer cells.

Table 1. BTK expression in primary tumours and metastases to lymph nodes.

Table 2. Spearman’s rho correlation between BTK level in tumour and node metastasis and selected parameters.

Figure 6. Schematic diagram of intracellular proteins BTK and JAK2 interaction and LFM-A13 mechanism of action. EPO: erythropoietin, LFM-A13: Bruton’s tyrosine kinase inhibitor; JAK2: non-receptor tyrosine kinase; BTK: Bruton’s tyrosine kinase; Akt: protein kinase B; PIP3: phosphatidylinositol-3,4,5-triphosphate; PH: pleckstrin homology domain.

Figure 6. Schematic diagram of intracellular proteins BTK and JAK2 interaction and LFM-A13 mechanism of action. EPO: erythropoietin, LFM-A13: Bruton’s tyrosine kinase inhibitor; JAK2: non-receptor tyrosine kinase; BTK: Bruton’s tyrosine kinase; Akt: protein kinase B; PIP3: phosphatidylinositol-3,4,5-triphosphate; PH: pleckstrin homology domain.