Anion inhibition studies of the Zn(II)-bound ι-carbonic anhydrase from the Gram-negative bacterium Burkholderia territorii

Figures & data

Table 1. Percentage of zinc and manganese in BteCAι as determined by atomic absorption spectroscopy.

Enzyme	Concentration (µM)	% Zn-BteCAι	% Mn-BteCAι
Zn-BteCAι – sample 1	33.5	98.30	1.70
Zn-BteCAι – sample 2	46.6	99.93	0.07
Mn-BteCAι – sample 1	20.0	97.85	2.15
Mn-BteCAι – sample 2	21.0	99.74	0.26

The buffer used for sample preparation reported 7.0 × 10⁻³ppm of Zn and 1.1 × 10⁻²ppm of Mn.

Table 2. Anion inhibition data of BteCAι as determined by a stopped-flow CO₂ hydrase assay.²⁰

Anion	KI (mM)^a
	hCA I^b	hCA II^b	E. coli β-CA^c	BteCAι^d
F^−	>300	>300	9.4	4.6
Cl^−	6	200	6.7	3.1
Br^−	4	63	3.8	4.8
I^−	0.3	26	>50	>50
CNO^−	0.0007	0.03	0.58	0.79
SCN^−	0.2	1.6	5.7	6.1
CN^−	0.0005	0.02	>50	>50
N3^−	0.0012	1.51	>50	>50
NO2^−	8.4	63	4.9	8.4
NO3^−	7	35	2.4	6.2
HCO3^−	12	85	0.81	0.94
CO3^2−	15	73	0.89	4.4
HSO3^−	18	89	3.7	8.4
SO4^2−	63	>200	1.7	5.8
HS^−	0.0006	0.04	2.7	6.2
NH2SO2NH2	0.31	1.13	0.011	0.086
NH2SO3H	0.021	0.39	0.0025	0.0062
PhAsO3H2	31.7	49	0.0061	0.008
PhB(OH)2	58.6	23	0.0028	0.009
ClO4^−	>200	>200	>50	>50
SnO3^2−	0.57	0.83	0.52	0.094
SeO4^2−	118	112	3.1	0.73
TeO4^2−	0.66	0.92	0.51	0,71
OsO5^2−	0.92	0.95	>50	>50
P2O7^2−	25.8	48	>50	>50
V2O7^2−	0.54	0.57	>50	>50
B4O7^2−	0.64	0.95	0.25	>50
ReO4^−	0.11	0.75	>50	>50
RuO4^−	0.101	0.69	9.5	>50
S2O8^2−	0.107	0.084	6.4	7.4
SeCN^−	0.085	0.086	3.1	6.6
NH(SO3)2^2−	0.31	0.76	1.5	>50
FSO3^−	0.79	0.46	0.83	9.3
CS3^2−	0.0087	0.0088	3.1	8.6
EtNCS2^−	0.00079	0.0031	0.084	0.81
PF6^−	>50	>50	>50	>50
CF3SO3^−	>50	>50	>50	>50

Inhibition of the human isoforms hCA I and II, and the bacterial β-CA from Escherichia coli are also shown for comparison.

^aMean from 3 different assays, by a stopped flow technique (errors were in the range of ± 5–10% of the reported values).

^bFrom Ref. ¹⁹.

^cFrom Ref. ²⁴.

^dThis work.

Khalifah RG. The carbon dioxide hydration activity of carbonic anhydrase. I. Stop-flow kinetic studies on the native human isoenzymes B and C. J Biol Chem 1971; 246:2561–73.

 PubMed Web of Science ®Google Scholar

(a) De Simone G, Supuran CT. (In)organic anions as carbonic anhydrase inhibitors. J Inorg Biochem 2012;111:117–29.

 PubMed Web of Science ®Google Scholar

(b) Bertucci A, Moya A, Tambutté S, et al. Carbonic anhydrases in anthozoan corals – a review. Bioorg Med Chem 2013;21:1437–50.

 PubMed Web of Science ®Google Scholar

Del Prete S, De Luca V, Nocentini A, et al. Anion inhibition studies of the beta-carbonic anhydrase from Escherichia coli. Molecules 2020;25:2564.

 PubMed Web of Science ®Google Scholar