A comparative study of carbonic anhydrase activity in lymphocytes from colorectal cancer tissues and adjacent healthy counterparts

Figures & data

Table 1. Clinical characteristics of CRC patients.

Patient ID	Sex	Age	Diagnosis	TNM	Stage
P1	M	71	Adenocarcinoma	–	–
P2	M	62	Adenocarcinoma	pT1a N0	I
P3	F	78	Adenocarcinoma	pT3 N0	IIa
P4	F	83	Adenocarcinoma	T3N0	2a
P5	M	78	Adenocarcinoma	pT3 N1b	IIIb
P6	M	58	Adenocarcinoma	pT3, pN1b	IIIb
P7	M	62	Adenocarcinoma	pT3 N0	IIa
P8	M	77	Adenocarcinoma	pT3 N0 M1	IVa
P9	F	53	Adenocarcinoma	pT1 N0	I
P10	M	85	Adenocarcinoma	pT3 N1b	IIIb
P11	F	85	Adenocarcinoma	T3N0	2a
P12	M	58	Adenocarcinoma	pT3, pN1b	IIIb
P13	M	84	Adenocarcinoma	pT2 N0	I
P14	M	74	Adenocarcinoma	pT3 N0	IIa
P15	F		Adenocarcinoma	–	–
P16	F	84	Adenocarcinoma	pT2 N1a	IIIa
P17	F	57	Adenocarcinoma	pT3 N0	IIa
P18	F	60	Adenocarcinoma	pT3, pN0	IIa

–, not detected.

Figure 1. Protonography of whole lymphocyte extracts prepared from tumour and adjacent healthy tissues as well as from peripheral blood of a patient with intestinal adenocarcinoma included in this study. The gel was run under denaturing but non-reducing conditions. Protonography showed a yellow band migrating with a rough molecular mass of about 26 kDa, which corresponds to the hydratase activity. Legend: Lane Std, molecular markers (from bottom to the top: 25 kDa, 37 kDa, 50 kDa, and others); lane 1, commercial bovine CA used as positive control; lane 2, lymphocyte collected from the tumour tissue; lane 3, lymphocyte collected from adjacent healthy tissues; lane 4, lymphocyte collected by PBMCs.

Table 2. Protein identification details of gel portions associated with CO₂ hydratase activity through protonography.

Sample	Accession	Gene name	Description	Sequence coverage (%)	Peptides (number)	PSMs	Unique Peptides	Amino acids	Score (Mascot)
Patient P1-HM	E5RHP7	CA1	Human carbonic anhydrase I	22	4	10	4	251	382
	P22748	CA4	Human carbonic anhydrase IV	9	2	4	2	312	134
	V9HW21; P00918	CA2	Human carbonic anhydrase II	32	6	18	6	260	502
Patient P1-CT	V9HW21; P00918	CA2	Human carbonic anhydrase II	3	1	1	1	260	45
Patient P1-PMBCs	E5RHP7	CA1	Human carbonic anhydrase I	39	7	18	7	251	627
	V9HW21; P00918	CA2	Human carbonic anhydrase II	25	5	9	5	260	379