Association study between variants in LHCGR DENND1A and THADA with preeclampsia risk in Han Chinese populations

Abstract

Objective: To evaluate the association between preeclampsia and three single nucleotide polymorphisms (rs13405728 in LHCGR gene; rs13429458 in THADA gene, and rs2479106 in DENND1A gene) which were identified to be genetic variants of polycystic ovary syndrome (PCOS) by genome-wide association study in Han Chinese populations.

Methods: A total of 784 northern Han Chinese women (378 controls and 406 cases) were genotyped for the three genetic variants by polymerase chain reaction and direct sequencing. Unconditional logistic regression analysis was used to adjust the impact of prepregnancy body mass index, primiparas, and maternal age.

Results: No significant difference was found in the allele frequencies of the three genetic variants between cases and controls (p > .05), but genotype frequency of the SNP rs2479106 was significantly differ between cases and controls when analyzed under recessive models (p = .02). There was also a substantial difference in the genotype frequencies of the SNP rs13429458 between cases and controls under additive models (p = .01).

Conclusions: Genetic variants of PCOS (rs13405728 in LHCGR gene; rs13429458 in THADA gene and rs2479106 in DENND1A gene) may not be involved in the development of preeclampsia in Han Chinese women.

Keywords:

• Preeclampsia
• PCOS: genetic variants
• association study

Acknowledgments

We are grateful to Lei Cheng, Yue-Hong Bian, and Guang-Yu Li of Shandong Provincial Hospital Affiliated to Shandong University for technical support. We especially thank all the patients for participating in this study.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: this study was supported by National Natural Science Foundation of China for Emergency Management projects [81741037]; National Natural Science Foundation of China [81571461, 81671434] Natural Science Foundations of Shandong Province [ZR2017BH046, ZR201702200727] and Medicine and Health Science and Technology Development Plan of Shandong Province [2016WS0411, 2016WS0412].