Integrative systematic revision of a new genus of Eumolpinae (Coleoptera: Chrysomelidae) endemic to New Caledonia: Dematotrichus gen. nov. and its numerous new hairy species

Figures & data

Table 1. Samples, including the species code number in Papadopoulou et al. (2013), JGZC voucher number and provenance of specimens yielding mtDNA sequences (with accession numbers) for phylogenetic assessment in Dematotrichus gen. nov.

Species	Sp. code	Voucher no.	Locality	cox1	rrnS
Ingroup (Dematotrichus)
D. capillaris sp. nov.	43	NC138	Baie Ugué	HF921536	HF920740
D. capillosus sp. nov.		4850	N Pic Vincent	–	OW496001
D. comans sp. nov.		4854	trail to Dogny	OW496057	–
D. comatulus sp. nov.	52	NC655	Aoupinié	HF921582	HF920783
	52	NC656	Aoupinié	HF921583	HF920784
	52	NC659	Aoupinié	HF921584	HF920785
	52	NC660	Aoupinié	HF921585	HF920786
	52	NC856	Aoupinié	OW496058	OW496002
	52	NC857	Aoupinié	OW496059	OW496003
	52	NC858	Aoupinié	OW496060	OW496004
D. crinitus sp. nov.	55	NC803	Mont Do	HF921579	HF920781
		4839	Mont Do	OW496061	OW496005
		4840	Mont Do	OW496062	OW496006
		4864	Mont Do	OW496063	OW496007
D. hirsutus sp. nov.	57	NC147	Station Beaupré	HF921602	HF920804
	57	NC768	Station Beaupré	HF921603	HF920805
	57	NC769	Station Beaupré	HF921604	HF920806
	57	NC770	Station Beaupré	HF921605	HF920807
	57	NC794	Station Beaupré	–	HF920808
	57	NC795	Station Beaupré	–	HF920809
	57	NC851	Station Beaupré	HF921606	HF920810
	57	NC852	Station Beaupré	–	HF920811
	57	NC853	Station Beaupré	HF921607	HF920812
	57	NC854	Station Beaupré	HF921608	HF920813
D. hirtus sp. nov.	50	NC107	Col d'Amieu	HF921586	HF920787
		4846	Col d'Amieu	OW496064	–
		4863	Col d'Amieu	OW496065	–
	50	NC111	Farino, sawmill	HF921587	HF920788
	50	NC125	Farino, sawmill	HF921588	HF920789
	50	NC463	Farino, sawmill	HF921589	HF920790
	50	NC464	Farino, sawmill	HF921590	HF920791
	50	NC556	Farino, sawmill	HF921591	HF920792
	50	NC557	Farino, sawmill	HF921592	HF920793
	50	NC558	Farino, sawmill	–	HF920794
	50	NC560	Farino, sawmill	HF921593	HF920795
	50	NC628	Farino, sawmill	HF921594	HF920796
	50	NC629	Farino, sawmill	HF921595	HF920797
	50	NC630	Farino, sawmill	HF921596	HF920798
	50	NC631	Farino, sawmill	HF921597	HF920799
	50	NC668	Farino	HF921598	HF920800
	50	NC669	Farino	HF921599	HF920801
	50	NC670	Farino	HF921600	HF920802
	50	NC671	Farino	HF921601	HF920803
	51	NC134	Pomea Mts.	HF921580	–
	51	NC591	Pomea Mts.	HF921581	HF920782
D. horridus sp. nov.		4855	Monts Koghi	OW496066	OW496008
D. pilosus (Jolivet, Verma & Mille)	56	NC025	Farino	HF921744	HF920955
	56	NC028	Farino	HF921745	HF920956
D. pubescens sp. nov.	53	NC796	Station Beaupré	–	HF921198
	53	NC797	Station Beaupré	–	HF921199
	53	NC798	Station Beaupré	–	HF921200
	53	NC838	Station Beaupré	–	HF921201
	53	NC839	Station Beaupré	HF921984	HF921202
D. setosus sp. nov.		4856	Col des Roussettes	OW496067	OW496009
D. villosus sp. nov.	54	NC850	Col d'Amieu	HF921575	HF920778
		4853	Camp de la Houe	OW496068	OW496010
Outgroups
Atrichatus ochraceus Sharp		4307	NZ: SI, Cable Bay Vines	OV133280	OV133253
Thasycles cordiformis Chapuis	48	NC034	Col d'Amieu	HF921573	HF920776
T. fuscus (Jolivet, Verma & Mille)	47	NC151	Col d'Amieu	HF921729	HF920941
T. laboulbenei (Montrouzier)	46	NC493	Pouebó	HF921737	HF920949

Fig. 1. Maximum likelihood tree of cox1 and rrnS data obtained from specimens morphologically similar to Dematochroma pilosa Jolivet, Verma & Mille and ascribed to the new genus Dematotrichus gen. nov. in this work. Bootstrap support numbers above 70% are shown next to the respective node and terminals are labelled with the species names proposed in this work. Asterisks identify when these specimens are selected as species holotypes. The inset shows an optimal ML tree with a single representative of the species considered in this work (voucher no. of specimen selected in brackets), except for D. hirtus sp. nov., paraphyletic for mtDNA, and it is treated as the best current hypothesis for the species tree of this group.

Table 2. Results of species delimitation within Dematotrichus gen. nov. based on an optimal ML topology obtained from mtDNA sequence data and the implementation of a Poisson Tree Processes branching model. The likelihood and posterior probabilities for data partitioning produced identical supports and are given together with the grouped samples, represented by JGZC voucher numbers.

Species	Support	Partition
1	1.000	NC138
2	0.981	NC850, 4853
3	0.912	NC803, 4839-4840, 4864
4	1.000	4856
5	1.000	4850
6	0.980	NC025, NC028
7	1.000	4855
8	1.000	4854
9	0.908	NC147, NC768-NC770, NC794-NC795, NC851-NC854
10	0.844	NC796-NC798, NC838-NC839
11	0.853	NC107, NC111, NC125, NC134, NC463-NC464, NC556-NC558, NC560, NC591, NC628-NC631, NC655-NC656, NC659-NC660, NC668-NC671, NC856-NC858, 4863, 4846

Fig. 2. Dorsal views of the male holotype and one female paratype of Dematotrichus hirtus sp. nov. (a), the male holotype of D. pubescens sp. nov. (b), the male holotype of D. capillaris sp. nov. (c), the male holotype and one female paratype of D. comatulus sp. nov. (d), and the male holotype and one female paratype of D. villosus sp. nov. (e).

Fig. 3. Lateral and apical dorsal views of the penises of Dematotrichus capillaris sp. nov. (a), D. comans sp. nov. (b), D. comatulus sp. nov. (c), D. crinitus sp. nov. (d), D. hirtus sp. nov. (e), D. villosus sp. nov. (f), D. pubescens sp. nov. (g) and D. hirsutus sp. nov. (h). All penises drawn at the same scale.

Fig. 4. Distribution maps of the species of Dematotrichus gen. nov.

Fig. 5. Dorsal views of the male holotype and one female paratype of Dematotrichus hirsutus sp. nov. (a), the male holotype and one female paratype of D. crinitus sp. nov. (b), the female holotype of D. capillosus sp. nov. (c), the male holotype of D. comans sp. nov. (d), the female holotype of D. horridus sp. nov. (e), and the female holotype of D. setosus sp. nov. (f).

Fig. 6. Spermathecae of Dematotrichus capillosus sp. nov. (a), D. comatulus sp. nov. (b), D. crinitus sp. nov. (c), D. hirsutus sp. nov. (d), D. hirtus sp. nov. (e), D. horridus sp. nov. (f), D. pilosus (Jolivet, Verma & Mille) (g), D. setosus sp. nov. (h), and D. villosus sp. nov. (i). All spermathecae drawn at the same scale.

Fig. 7. Dorsal (a) and lateral (b) views of the male holotype of Dematotrichus pilosus (Jolivet, Verma & Mille) and accompanying labels (c).

Papadopoulou, A., Cardoso, A., & Gómez-Zurita, J. (2013). Diversity and diversification of Eumolpinae (Coleoptera: Chrysomelidae) in New Caledonia. Zoological Journal of the Linnean Society, 168(3), 473–495. https://doi.org/10.1111/zoj.12039

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