https://orcid.org/0000-0002-5881-6796
Figures & data
Table 1. Genetics of selected inherited cardiovascular diseases.
Figure 1. Graphical description of the prime editing process.
(a) The prime editor machine is composed of a Cas9/RT complex protein and pegRNA. The Cas9 nickase-RT fusion works together on the target loci to cut the PAM strand. The location of the PAM is indicated by the red DNA bases and scissors. (b) The resulting 3′-overhang can be primed by RT on the RTT template to install the edition on a newly synthesized DNA strand. (c) There is an equilibration between 3′-flap and 5′-flap, however, (d) 3′-flap insertion into new heteroduplex DNA might be fixed into the genome by further repair. The details of each strategy have been discussed in the text.
Figure 2. Prime editing for genetic cardiac disorders. (a) Viral and non-viral delivery methods can be employed in “all-in-one” or in dual vector systems to transfer PEs into the nucleus of cardiac cells. (b) The scope of prime editing for correction of cardiac genetic disorders. Prime editing can cover the treatment of nearly all possible genetic defects by nucleotide mutations, sequence disruptions, exon deletions, and even insertions.
