Figures & data
(a) The prime editor machine is composed of a Cas9/RT complex protein and pegRNA. The Cas9 nickase-RT fusion works together on the target loci to cut the PAM strand. The location of the PAM is indicated by the red DNA bases and scissors. (b) The resulting 3′-overhang can be primed by RT on the RTT template to install the edition on a newly synthesized DNA strand. (c) There is an equilibration between 3′-flap and 5′-flap, however, (d) 3′-flap insertion into new heteroduplex DNA might be fixed into the genome by further repair. The details of each strategy have been discussed in the text.