Abstract
Extracts obtained from Cyrtocarpa procera Kunth were evaluated to determine their toxicity on Artemia salina Leach. All the extracts showed a significant activity. Bioguided fractionation of these extracts led to the isolation of β-sitosterol (1) and the identification of 1,3-propyl-dipentadecanoate (2), 3-hydroxypropyl-9-octadecenoate (3), pentadecylbenzene (4), eicosylbenzene (5), docosane (6), heptacosane (7), dotriacontane (8) and 2,6,10-trimethyltetradecane (9) by GC–MS.
Acknowledgements
This study was financed by a grant of CONACyT (SEP-2003-CO2-43440) and PROMEP-SEP. The authors would like to thank Raunel Tinoco, IBT-UNAM, for recording the GC–MS. Special thanks to Manases González and María Gregoria Medina for recording the NMR, MS, UV and IR spectra. The authors are grateful to Dr Rafael Villalobos Molina, CINVESTAV-IPN sede sur, México, for providing facilities to determine the pharmacological evaluation and to Juan J. López Guerrero and Daniel Godínez for their technical assistance.