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Natural Product Research
Formerly Natural Product Letters
Volume 20, 2006 - Issue 9
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Original Articles

ELISA and LC-MS/MS methods for determining cyanobacterial toxins in blue-green algae food supplements

M. Bruno Department of Environment and Primary Prevention, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161, Rome, ItalyCorrespondence[email protected]
,
M. Fiori Department of Food and Animal Safety, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161, Rome, Italy
,
D. Mattei Department of Environment and Primary Prevention, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161, Rome, Italy
,
S. Melchiorre Department of Environment and Primary Prevention, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161, Rome, Italy
,
V. Messineo Department of Environment and Primary Prevention, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161, Rome, Italy
,
F. Volpi Department of Environment and Primary Prevention, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161, Rome, Italy
,
S. Bogialli Department of Chemistry, University of Rome “La Sapienza”, P.le Aldo Moro 1, 00161, Rome, Italy
&
M. Nazzari Department of Chemistry, University of Rome “La Sapienza”, P.le Aldo Moro 1, 00161, Rome, Italy
 show all
Pages 827-834 | Accepted 12 Oct 2005, Published online: 23 Aug 2006
 
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Abstract

The use of natural products as a diet supplement is increasing worldwide but sometimes is not followed by adequate sanitary controls and analyses. Twenty samples of pills and capsules of lyophilised cyanobacteria (blue-green algae), commercialised in Italy as dietary supplements, were found positive at the Vibrio fischeri bioassay. Further analyses with ELISA and LC-MS/MS methods revealed the presence of four microcystin (MC) analogues, MC-LR, -YR, -LA, -RR and two demethylated forms of MC-RR. The highest total microcystin content was 4.5 and 1.4 µg g−1 in pills and capsules, respectively. The ELISA measurements, compared to the LC-MS/MS analyses, showed significantly lower concentrations of microcystins in pills, this confirming a possible ELISA underestimate of mixed microcystins, due to different sensitivities for some toxic analogues.

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