Bioactivities and chemical constituents of a Vietnamese medicinal plant Che Vang, Jasminum subtriplinerve Blume (Oleaceae)

Abstract

Five crude extracts were made from leaves and stems of Jasminum subtriplinerve Blume (Oleaceae) and investigated for antimicrobial, antioxidant and cytotoxic activities. The extractions were done with petroleum ether, ethyl acetate, ethanol, methanol or water. All extracts exhibited anti-bacterial activity except the water fraction. On the other hand, all extracts exhibit antioxidant activity except the petroleum ether fraction using the DPPH radical scavenging assay. Only the petroleum ether fraction showed a cytotoxicity activity against tested cell-lines, Hep-G2 and RD with IC₅₀ values of 19.2 and 20 µg mL⁻¹, respectively. From the petroleum ether and ethyl acetate extracts, two triterpenes namely 3β-acetyl-oleanolic acid and lup-20-en-3β-ol and a sterol, stigmast-5-en-3β-ol were isolated. The structure of those compounds were elucidated by spectrometric methods IR, MS, 1D-NMR, 2D-NMR and simulated ACD/NMR spectra. The data presented here indicate that J. subtriplinerve do contain compounds with interesting biological activity.

Keywords:

• Jasminum suptriplinerve
• Oleaceae
• Triterpenes
• Cytotoxicity
• Antimicrobial
• Antioxidant
• MIC value
• IC₅₀ value

Acknowledgement

This work was supported by a grant from DANIDA via the ENRECA program (Denmark) for Ms Dai Hue Ngan. The authors are grateful to Dr Chu Dinh Kinh (Institute of Chemistry, VAST, Hanoi) for the mass and NMR spectra. We thank to Dr Tran Hung (Department of Pharmacognosy, School of Pharmacy, HCMC) for his helpful advice.