CPT accumulation in the fruit and during early phases of plant development in Camptotheca acuminata Decaisne (Nyssaceae)

Abstract

We describe the dynamics of camptothecin (CPT) accumulation during the early phases of plant development in Camptotheca acuminata. TLC and HPLC analyses were performed on the entire mature fruit and all of its parts and on the organs of seedlings in different developmental stages. In the mature fruit, the CPT content was relatively high (2.83 mg g⁻¹ DW); it was accumulated especially in the endosperm (1.82 mg g⁻¹ DW) and embryo (1.10 mg g⁻¹ DW). Regarding seedlings, CPT was present in all of the organs, at all developmental stages. In the cotyledons, content was greatest in the early developmental stages (4.23 mg g⁻¹ DW) and drastically decreased once the organ reached maturity (0.34 mg g⁻¹ DW). A similar trend was observed for the true leaves. The results suggest that CPT is translocated from the organs at an advanced developmental stage to those at an early stage.

Keywords:

• Monoterpene indole alkaloids
• Camptotheca acuminata
• Camptothecin