Anti-inflammatory activity of an alkaloid from Solanum trilobatum on acute and chronic inflammation models

Abstract

The solasodine isolated from Solanum trilobatum has been examined for possible anti-inflammatory activity in acute and chronic inflammatory animal models. Solasodine (5, 30 and 75 mg kg⁻¹) exerted statistically significant and dose-dependent anti-inflammatory activity in carrageenan-induced rat paw oedema, which was similar to that of indomethacin (10 mg kg⁻¹), a known anti-inflammatory agent. At the dose of 75 mg kg⁻¹, solasodine inhibited the arachidonic acid-elicited rat paw oedema 1 h after arachidonic acid challenge. Topical application of solasodine significantly inhibited the ear inflammation induced by multiple applications of tetradecanoyl-phorbol 13-acetate. It also suppressed the volume of exudates, total leucocytes and amount of neutrophil migration into the rat pleural cavity. Administration of solasodine at a dose of 75 mg kg⁻¹ significantly inhibited the adjuvant-induced rat paw oedema. These results suggest that solasodine exerts anti-inflammatory activity, at least partly through the inhibition of cyclooxygenase and 5-lipoxygenase pathways.

Keywords:

• solasodine
• carrageenan
• arachidonic acid
• tetradecanoyl-phorbol acetate
• complete-Freund's adjuvant

Acknowledgements

We thank the following people at SAIF, CDRI Lucknow for providing the analytical data of the compounds reported in this report: Prof. Raja Roy, Mr Harsh Mohan Gauniyal for the NMR experimental work and Mr Sunil Arnold Singh for the mass spectral data.