Steroids and phenolic constituents from the fruiting bodies of the basidiomycete Sarcodon joedes

Abstract

Nine secondary metabolites, including four steroids, four phenolics and one cerebroside, were isolated from the methanol extract of the fruiting bodies of the basidiomycete Sarcodon joedes. The isolated compounds were identified by spectroscopic analyses as (22E,24R)-6β-methoxyergosta-7,22-diene-3β,5α-diol (1), 2′,3′-diacetoxy-3,4,5′,6′,4″-pentahydroxy-p-terphenyl (2), cerebroside B (3), ergosta-7,22-dien-3β-ol (4), ergosterol peroxide (5), (22E,24R)-3β-hydroxy-ergosta-5,22-dien-7-one (6), benzoic acid (7), methyl p-hydroxybenzoate (8) and 3,4-dihydroxybenzoic acid (9). The cytotoxic activities of these compounds were evaluated. All these compounds were isolated from this fungus for the first time.

Keywords:

• basidiomycete
• Sarcodon joedes
• steroid
• p-terphenyl
• cytotoxicity
• natural products

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 30370019, 30670221, 30770237, and 30971882) and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry. The authors also are grateful to Prof. K. Ding at the Glycochemistry & Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences for helping with activity measurements.