A new triterpenoid saponin from Gleditsia sinensis and its antiproliferative activity

Abstract

Chemical investigation of the anomalous fruits of Gleditsia sinensis led to the isolation and identification of a new triterpenoid saponin, 3-O-β-D-xylopyranosyl-(1 → 2)-α-L-arabinopyranosyl-(1 → 6)-β-D-glucopyranosyl oleanolic acid 28-O-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyrano--syl-(1 → 4)-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 3)-β-D-glucopyranosyl ester (1), along with other nine known compounds (2–10). All the isolates from this species were reported for the first time. The structure of Compound 1 was determined by a detailed analysis using various analytical techniques, including 1D and 2D NMR. In vitro antiproliferative activities of Compound 1 on MCF-7 and Hep-G2 tumor cell lines were evaluated. IC₅₀ values against the two cell lines were 9.5 and 11.6 μM, respectively.

Keywords:

• Gleditsia sinensis
• triterpenoid saponin
• antiproliferative

Acknowledgement

Thanks to Dr Mark Smith, National Research Council of Canada for the improvement of the manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

This work was supported by the National Science and Technology Support Program of China [grant number 2015BAD16B02]; the Youth Foundation of Shandong Academy of Agricultural Sciences [grant number 2015YQN57].

Additional information

Funding

This work was supported by the National Science and Technology Support Program of China [grant number 2015BAD16B02]; the Youth Foundation of Shandong Academy of Agricultural Sciences [grant number 2015YQN57]; the Shandong Taishan overseas scholars introduction programme (tshw20120747).