A novel Salvialactomine from the callus culture of Salvia santolinifolia Boiss

Abstract

A novel compound Salvialactomine (1) along with two other unusual occurring natural products Pentatriacontanoic acid 1, 3-dihydroxypropyl ester (2) and 5-Methylflavone (3) were isolated from the callus of Salvia santolinifolia Boiss. Callus was initiated on MS medium containing NAA (0.5 mg/L) and further sub-cultured on MS medium supplemented with NAA with BA (0.5 + 1.5 mg/L). The structures of isolated compounds were determined by using mass spectrometry, 1D, and 2D–NMR techniques. Compounds 1, and 3 were tested for two different cancer cell lines, i.e. Hela (Cervical cancer cell) and PC-3 (Prostate cancer cells). IC₅₀ was found as > 30 using Doxorobicin (0.912 ± 0.12 μmol L⁻¹) as a standard.

Keywords:

• Secondary metabolites
• callus
• Salvia santolinifolia
• lamiaceae
• hela
• PC-3
• Murashige & Skoog (MS)
• 1-napthalen acetic acid (NAA)
• 6-benzyladenine (BA)

Acknowledgements

This study is a part of Tour Jan PhD thesis. We are thankful to the University of Karachi and HEJRICCBS for providing technical and instrumental support.