Phytochemical studies of Jurinea macrocephala roots from Western Himalaya

Abstract

Phytochemical investigation of the Jurinea macrocephala roots afforded six compounds namely β-sitosterol (1), lupenone (2), physcion (3), ptiloepoxide (4), 20, 21α-epoxytaraxastan-3β-ol (5) and chlorogenic acid (6). All the compounds were isolated for the first time in roots. The structures of the compounds were established by analysis of their spectroscopic (¹H and ¹³C NMR) and spectrometric (MS) data, as well as by comparison of these with those reported in the literature. Metabolic profiling of chloroform and ethyl acetate fractions were also accomplished using NMR. In NMR analysis, ERETIC (electronic reference to access in-vivo concentration) 2 method was used for the quantification of identified metabolites. High quantity of chlorogenic acid (6, 130 mg/g) lupenone (2, 33.4 mg/g) and amyrins (α, β) (170.6 mg/g) were detected in ethyl acetate and chloroform fractions. Further studies on the biological evaluation of phenolic-rich and chloroform fractions could be beneficial to explore its pharmaceutical potential.

Graphical Abstract

Keywords:

• Jurinea macrocephala
• NMR
• ERETIC 2
• chlorogenic acid

Acknowledgement

The authors gratefully acknowledge Dr. Sanjay Kumar, Director, CSIR-IHBT Palampur (HP), India, for continuous encouragement and for providing the necessary facilities during the course of the study. Thanks are also due to UGC for awarding senior research fellowship to Mr. Ashish Kumar. Authors are also grateful to CSIR, New Delhi for funding the BSC-0106 project under which the work was carried out.

Disclosure statement

No potential conflict of interest was reported by the authors.