A new ocotillol-type ginsenoside from stems and leaves of Panax quinquefolium L. and its anti-oxidative effect on hydrogen peroxide exposed A549 cells

Abstract

A new ocotillol-type ginsenoside, namely 12-one-pseudoginsenoside F₁₁ (12-one-PF₁₁), was isolated from stems and leaves of Panax quinquefolium, whose structure was elucidated 6-O-[α-L-rhamnopyranosyl-(1-2)-β-D-glucopyranosyl]-dammar-12-one-20S,24R-epoxy-3β,6α,25-triol. 12-one-PF₁₁ significantly suppressed hydrogen peroxide induced oxidative stress in human lung carcinoma A549 cells. As compared with model group, 12-one-PF₁₁ improved cell viability of A549 cells in a dose-dependent manner, and significantly decreased the generation of malondialdehyde (MDA) and increased production of superoxide dismutase (SOD) and glutathione (GSH) and protein expression levels of nuclear related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in A549 cells.

Graphical Abstract

Keywords:

• 12-one-pseudoginsenoside F₁₁
• Panax quinquefolium L.
• A549 cells
• antioxidant activity
• Nrf2/HO-1

Acknowledgments

The authors are very grateful to financial support from the Talents Team Major Program of Jilin Province of China (JRCBTZ. [2016] No. 3).

Disclosure statement

The authors declared no conflict of interests.

Additional information

Funding

The authors are very grateful to financial support from the Talents Team Major Program of Jilin Province of China (JRCBTZ. [2016] No. 3)