Antifungal activity of Bignoniaceae plants on Aspergillus carbonarius and Aspergillus niger

Abstract

Twenty four extracts from Bignoniaceae plants of northwest Argentina were tested for antifungal activity against Aspergillus species responsible of the grape black rot. Stems and leaves of Amphilophium cynanchoides, Macfadyena cynanchoides, Tecoma stans and Jacaranda mimosifolia were separately extracted with solvents of increasing polarity to obtain the dichloromethane (fCH₂Cl₂), ethyl acetate (fEtOAc) and methanol extracts (fMeOH). The fCH₂Cl₂ from stem of M. cynanchoides had the lowest IC₅₀ (1.0–1.2 mg/mL) and MID values (0.6–1.2 mg) and the highest ID values (5.0–6.8 mm) on A. niger and A. carbonarius. The main contributors of the antifungal activity of fCH₂Cl₂ were identified as lapachol (MIC = 0.25–1.00 mg/ml) and 1-hydroxy-4-methylanthraquinone (MIC = 0.0625–0.125 mg/mL). These compounds synergized the antifungal activity of sodium metabisulfite and showed an additive effect in mixtures with propiconazol. They might be used as additives of commercial antifungals to protect grapes against A. niger and A. carbonarius.

Graphical Abstract

Keywords:

• Aspergillus
• Bignoniaceae
• black rot

Disclosure statement

No potential conflict of interest was reported by the author(s).