Exophiarin, an isocoumarin from the fungus Exophiala sp. with antihyperglycemic activity

Abstract

Chemical characterization of ethyl acetate extract of Exophiala sp. has afforded the isolation of three compounds including a new isocoumarin named exophiarin (1). Exophiala sp. was obtained from the soil containing dumped organic waste (litter). Initially, LC-UV-MS analysis of the extract of Exophiala sp. revealed the presence of a new compound having molecular weight 438 (1) and previously reported TPI-2 and TPI-5. The novelty was established using advanced database search comprising of biological source, molecular weight and UV profile. 1D, 2D NMR and HRMS data have been used for structure elucidation. Exophiarin with TPI-2 and TPI-5 have displayed moderate improvement in glucose uptake activity when tested in rat skeletal muscle cell line L6.

Graphical Abstract

Keywords:

• Isocoumarin
• Exophiala sp.
• LC-MS
• dereplication
• fungus
• exophiarin
• glucose uptake assay

Acknowledgement

We acknowledge Rajendra Kshirsagar, Piramal Enterprises Limited for NMR support.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

Funding was provided by Piramal Enterprises Limited, Mumbai.