Anti-inflammatory and cytotoxic agents from Xylaria sp. SWUF09-62 fungus

Abstract

The ongoing search for anti-cancer agents from microorganisms led to the isolation of four new compounds including 6-ethyl-8-hydroxy-4H-chromen-4-one (1), 6-ethyl-7,8-dihydroxy-4H-chromen-4-one (2), (3S)-3,4-dihydro-8-hydroxy-7-methoxy-3-methylisocoumarin (3) and (3S)-3,4-dihydro-5,7,8-trihydroxy-3-methylisocoumarin (4), together with eleven known compounds (5–15) from Xylaria sp. SWUF09-62 fungus. The chemical structures were deduced from IR, 1D and 2D NMR, and MS data. The absolute configurations of 3 and 4 were determined by ECD experiment. Compounds 2 and 4 indicated possible chemo-prevention and chemo-therapeutic properties, exhibited anti-inflammatory properties by reducing nitric oxide production in LPS-stimulated RAW264.7 cells (IC₅₀ = 1.57 ± 0.25 and 3.02 ± 0.27 μg/mL) and cytotoxicity against HT29 cells (IC₅₀ = 16.46 ± 0.48 and 97.78 ± 7.14 μg/mL).

GRAPHICAL ABSTRACT

Keywords:

• Anti-inflammatory
• chromone
• cytotoxicity
• mellein
• nitric oxide (NO)
• Xylaria

Acknowledgements

Financial support from the Office of National Research Council of Thailand (NRCT) and the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research and Innovation is gratefully acknowledged.

Disclosure statement

No potential conflict of interest was reported by the authors.