Discovery and evaluation on the antibacterial and cytotoxic activities of a novel antifungalmycin N2 produced from Streptomyces sp. strain N2

Abstract

Antifungalmycin N2 (3-methyl-3,5-amino-4-vinyl-2-pyrone, C₆H₇O₂N) was a novel metabolite produced from Streptomyces sp. strain N2, and the present study aimed to evaluate its antibacterial and cytotoxic properties. By using Oxford cup method, the obtained results revealed that antifungalmycin N2 exhibited a significant antibacterial activity against the pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, and Micrococcus kristinae, especially the Gram-positive S. aureus. Meanwhile, the MTT assay showed that antifungalmycin N2 could exert a marked inhibitory action on tumor cell lines, such as the cell lines of BEL-7402 (human hepatocellular carcinoma), Hela (human cervical carcinoma), HCT116 (human colon cancer), and SW620 (human colon cancer). And the IC₅₀ values antifungalmycin N2 against the above cell lines ranged from 11.23 to 15.37 μg/mL. In conclusion, the antibacterial and cytotoxic activities suggested that the novel antifungalmycin N2 was a promising active structure to be developed as new drug for treating infectious diseases and cancers.

GRAPHICAL ABSTRACT

Keywords:

• Antifungalmycin N2
• antibacterial activity
• cytotoxic activity; Streptomyces sp. strain N2

Disclosure statement

All the authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Funding

This work was financially supported by the National Natural Science Foundation of China (Grant No. 31760546), and the Training Program for Young Scientists of Jiangxi Provincial Department of Science and Technology (20142BCB23025).