Abstract
The objective was to investigate the role of consumption of grape juice (GJ), red wine (RW) or resveratrol solution (RS) on rats treated with a high-fat diet (HFD). Among the drinks offered, GJ had lower content of polyphenols and trans-resveratrol. Nevertheless, GJ showed similar content of anthocyanin and antioxidant activity to RW, although higher than RS. In rats treated with HFD, consumption of GJ presented best antioxidant and anti-inflammatory activity, reducing glutathione peroxidase and interleukin-6 serum levels. In addition, GJ promoted better levels of cholesterol and liver markers. On the other hand, RW aggravated the oxidizing effect of HFD, increasing catalase activity and interleukin-6 level. Already, RS showed no benefit in animals. Thus, GJ minimized the effects of HFD on oxidative stress and inflammation beyond promoted better levels of lipid profile and liver biomarkers. However, consumption of RS showed no benefit and RW revealed a pro-oxidant effect, as did HFD.
Disclosure statement
No potential conflict of interest was reported by the authors.
Figure 1. Dietary protocol. Experimental model: (CG) Control group supplemented with control feed and water; (HG) High-fat diet group received high-fat diet and water; (JG) Grape juice group represents animals submitted to high-fat diet treated with grape juice (15 mL/day) and water; (WG) Wine group represents animals submitted to high-fat diet treated with red wine (10 mL/day) and water; (RG) Resveratrol group represents animals submitted to high-fat diet treated with resveratrol solution isolated (15 mL/day – 40 mg/L) and water. Diets and solutions were administered during a period of 60 days.
![Figure 1. Dietary protocol. Experimental model: (CG) Control group supplemented with control feed and water; (HG) High-fat diet group received high-fat diet and water; (JG) Grape juice group represents animals submitted to high-fat diet treated with grape juice (15 mL/day) and water; (WG) Wine group represents animals submitted to high-fat diet treated with red wine (10 mL/day) and water; (RG) Resveratrol group represents animals submitted to high-fat diet treated with resveratrol solution isolated (15 mL/day – 40 mg/L) and water. Diets and solutions were administered during a period of 60 days.](/cms/asset/ff818036-57ff-49fd-b117-ff3ca2a28817/gnpl_a_1747458_f0001_b.jpg)
Figure 2. Antioxidant capacity, total phenolic, antocianins and trans-resveratrol content of (GJ) Grape juice; (RW) Red wine; (RS) Resveratrol solution. Means significant difference with different letters (p < 0.05). Abbreviations: (GAE) Gallic acid equivalents; (mg) milligrams; (L) Liter; (DPPH) 2,2- difenil-1-picrilhidrazil; (FRS) Free radical scavenging; Statistical significance was determined by ANOVA followed by Tukey’s post hoc multiple mean comparison test.
![Figure 2. Antioxidant capacity, total phenolic, antocianins and trans-resveratrol content of (GJ) Grape juice; (RW) Red wine; (RS) Resveratrol solution. Means significant difference with different letters (p < 0.05). Abbreviations: (GAE) Gallic acid equivalents; (mg) milligrams; (L) Liter; (DPPH) 2,2- difenil-1-picrilhidrazil; (FRS) Free radical scavenging; Statistical significance was determined by ANOVA followed by Tukey’s post hoc multiple mean comparison test.](/cms/asset/87cb6c07-d984-405c-8041-a48afdb141ae/gnpl_a_1747458_f0002_b.jpg)