A new biflavonoids from Aster tataricus induced non-apoptotic cell death in A549 cells

Abstract

A new biflavonoids, (2R,2''R)-7-O-methyl-2,3,2'',3''-tetrahydrorobustaflavone (1), along with five known flavonoids (2-6) were isolated from the MeOH extract of Aster tataricus. Among them, compounds 1-2 were the C-3'-C-6'' type biflavonoids obtained from the genus Aster for the first time. The structures and absolute configurations of compound 1 was confirmed based on extensive spectroscopic and circular dichroism analyses. Compound 1 exhibited moderate cytotoxicity against seven human cancer A549, HepG2, PC3, DU145, MCF-7, LOVO and NCI-H1975 cell lines. Compound 1 remarkably inhibited the proliferation of A549 cancer cells with IC₅₀ value of 5.4 μM. Further preliminary pharmacological study, 1 induces A549 cell death by non-apoptotic forms through flow cytometry and cell scratch assay data.

Graphical Abstract

Keywords:

• Aster tataricus
• biflavonoids
• A549

Acknowledgements

This work was supported by the Scientific Research Foundation of Hunan Provincial Education Department (20B415) and the Technology Project Foundation of Tongren ([2018]48).

Disclosure statement

No potential conflict of interest was reported by the authors.